In this study heavy metal concentrations of Fe, Pb and Zn in flesh and liver of tilapia fish were seasonally estimated in samples collected from Lake Nasser and Balana sewage plant at Aswan during 2013 and 2014. Also, water samples were obtained from the same investigated sites to measure the concentrations of the three investigated metals in addition to some physico-chemical parameters during the four seasons. The results indicated that in most cases the investigated metal concentrations in fish organs from Balana sewage plant were higher than those of fish organs from Lake Nasser and the safety baseline according to WHO. Also, the levels of the investigated metals, chemical and physical water parameters were higher in Balana sewage plant than those in Lake Nasser and Balana Nile branch.