Special photocatalytic activity, extreme availability, non-toxicity, biocompatibility, and low cost make TiO₂ nanoparticles mainly attractive for industrial of different high value-added produces. The present work aims of harnessing nanotechnology as one of the most important frontier sciences to develop the printing of natural fibers using the most ecologically friendly dyes, namely turmeric  dye  to confirm the sustainability of the environment for upcoming generations, decrease pollution resultant from textile printing, overcome the harms of using synthetic colorants, synthetic thickeners, and synthetic fibers on environmental balance and human healthiness. Environmental natural dye (turmeric dye) and the natural thickener (tamarind thickener) are used to produce a natural printing paste with a proper viscosity for printing natural fabrics by the flat silk screen printing. In this work, three diverse methods of treating fabrics were used, which are pre-treatment, simultaneous treatment, and post-treatment with TiO₂ nanoparticles at varying concentrations (0.5%, 1%, 1.5%, and 2% W.O.F). TiO₂ nanoparticles were prepared using sol-gel technique. The prepared nanoparticles were analyzed using Scanning electronic microscope(SEM), and transmission, electronic microscope(TEM), tensile strength and elongation, microbial protection, Color strength K/S values, fastness properties, and Ultraviolet protection factor (UPF were measured on fabric samples. Results presented that the K/S values of treated samples are higher than the original samples.  All treated fabrics have excessive fastness values for washing, perspiration, and rubbing. But all treated fabric printed with curcuma show low light fastness properties. Rise in the tensile strength of fabrics. The combination of these nanoparticles in the structure of the natural fabrics creates the ending product antimicrobial. The UPF values of all the treated printed fabric samples are upper than those of the blank ones. Development of the function and health properties and value of the resultant fabrics while keeping the ecological balance. By overcoming the lowly shadow and fastness properties of natural colorants by treating fabrics with green materials. Rise the durability of treated natural fabrics and the default service life. The printed fabrics obtained advance an antibacterial agent for Staphylococc            Special photocatalytic activity, extreme availability, non-toxicity, biocompatibility, and low cost make TiO₂ nanoparticles mainly attractive for industrial of different high value-added produces. The present work aims of harnessing nanotechnology as one of the most important frontier sciences to develop the printing of natural fibers using the most ecologically friendly dyes, namely turmeric  dye  to confirm the sustainability of the environment for upcoming generations, decrease pollution resultant from textile printing, overcome the harms of using synthetic colorants, synthetic thickeners, and synthetic fibers on environmental balance and human healthiness. Environmental natural dye (turmeric dye) and the natural thickener (tamarind thickener) are used to produce a natural printing paste with a proper viscosity for printing natural fabrics by the flat silk screen printing. In this work, three diverse methods of treating fabrics were used, which are pre-treatment, simultaneous treatment, and post-treatment with TiO₂ nanoparticles at varying concentrations (0.5%, 1%, 1.5%, and 2% W.O.F). TiO₂ nanoparticles were prepared using sol-gel technique. The prepared nanoparticles were analyzed using Scanning electronic microscope(SEM), and transmission, electronic microscope(TEM), tensile strength and elongation, microbial protection, Color strength K/S values, fastness properties, and Ultraviolet protection factor (UPF were measured on fabric samples. Results presented that the K/S values of treated samples are higher than the original samples.  All treated fabrics have excessive fastness values for washing, perspiration, and rubbing. But all treated fabric printed with curcuma show low light fastness properties. Rise in the tensile strength of fabrics. The combination of these nanoparticles in the structure of the natural fabrics creates the ending product antimicrobial. The UPF values of all the treated printed fabric samples are upper than those of the blank ones. Development of the function and health properties and value of the resultant fabrics while keeping the ecological balance. By overcoming the lowly shadow and fastness properties of natural colorants by treating fabrics with green materials. Rise the durability of treated natural fabrics and the default service life. The printed fabrics obtained advance an antibacterial agent for Staphylococcus aureus, and Escherichia coli , and ultraviolet protective. us aureus, and Escherichia coli , and ultraviolet protective.   TiO₂ Nanoparticles, UV Protective, Antibacterial Agent , Turmeric Dye, Green Treatment.