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IN SILICO DESIGN AND VALIDATION OF A NOVEL PCR-RFLP ASSAY FOR GPRC6A RS2247911 POLYMORPHISM INVOLVED IN MALE INFERTILITY

Article

Last updated: 25 Dec 2024

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Abstract

Male infertility is a prevalent disorder affecting approximately 7% of the male population, at least 2,000 genes are involved in spermatogenesis, availability of genetic investigations is a challenge in developing countries, GPRC6A, G-protein coupled receptor, activated by Osteocalcin (OCN)an osteoblast secreted hormone, is a master regulator of a complex metabolic and reproductive networks. rs2247911 in GPRC6A plays a  role in male fertility and glucose metabolism, Although DNA sequencing is regarded the golden standard tool for genotyping, it is less affordable in developing world laboratories, polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) assay is an inexpensive and highly valuable tool for genotyping, this study aimed to develop (PCR-RFLP) method for assessment of rs2247911 polymorphism.10% of male subjects genotyped previously by sequencing were genotyped the by PCR-RFLP method based on Geneious® bioinformatics tool suggested restriction enzyme (ScrFI), PCR-RFLP 100% correctly identified genotypes. we present for the first time an accurate and inexpensive method for genotyping rs2247911.
 

DOI

10.21608/bfsa.2022.271821

Keywords

GPRC6A, PCR-RFLP, Sequencing, Osteocalcin, testis failure

Authors

First Name

Kenda

Last Name

Jawich

MiddleName

-

Affiliation

Department of Biochemistry and Microbiology, Faculty of Pharmacy, Damascus University, Damascus, Syrian Arab Republic

Email

kindajawish1@gmail.com

City

-

Orcid

0000-0002-6471-6644

First Name

Marwan

Last Name

Alhalabi

MiddleName

-

Affiliation

Department of Embryology and reproductive Medicine, Faculty of Medicine, Damascus University, Damascus, Syrian Arab Republic

Email

profalhalabi@icloud.com

City

-

Orcid

-

First Name

Sahar

Last Name

Al Fahoum

MiddleName

-

Affiliation

Department of Biochemistry and Microbiology, Faculty of Pharmacy, Damascus University, Damascus, Syrian Arab Republic

Email

sahar_fahoum@yahoo.com

City

-

Orcid

-

Volume

45

Article Issue

2

Related Issue

37882

Issue Date

2022-12-01

Receive Date

2022-05-08

Publish Date

2022-12-01

Page Start

1,077

Page End

1,084

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 2_Pages 1077-1084.pdf

PDF . 1.1MB

Link

https://bpsa.journals.ekb.eg/article_271821.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=271821

Order

271,821

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

IN SILICO DESIGN AND VALIDATION OF A NOVEL PCR-RFLP ASSAY FOR GPRC6A RS2247911 POLYMORPHISM INVOLVED IN MALE INFERTILITY

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Type

Article

Created At

22 Jan 2023