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CHROMATOGRAPHIC ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF SOME CHEMICAL CONSTITUENTS OF PETROSELINUM CRISPUM LEAVES AND EVALUATING AS HEPATOPROTECTIVE AGENTS

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Last updated: 25 Dec 2024

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Abstract

Petroselinum crispum Mill. (family Apiaceae) is widely used as an essential spice due to its health benefits. This study aimed to investigate the constituents of P.crispum aerial parts and evaluate its hepatoprotective activity. Ethyl acetate and n-butanol fractions of P. crispum subjected to different chromatographic separation techniques. The compounds were identified on the basis of spectral data and compared with previously reported data. Also, the methanolic extract and n-butanol fraction were evaluated for in-vivo hepatoprotective activity against carbon tetrachloride (CCl4) induced hepatotoxicity in mice (10 groups; 8 mice/group). All groups intraperitonially injected by CCl4 (0.5mg/kg b.w., i.p.) in single dose at 11th day, the treated groups received (100, 200, 300, 400 mg/kg b.w.) of MeOH extract and BuOH fraction separately for 14 days. The results revealed that seven compounds were isolated from EtOAc and n-BuOH fractions which were identified as β-sitosterol (1), quercetin (2), isorhmentin (3), apigenin (4), apigenin-7-O-β-D-glucopyranoside (cosmosiin) (5), diosmetin-7-O-β-D-glucopyranoside (6), apigenin 7-O-β- D- apiofuranosyl (1→2)-β- D glucopyranoside (apiin) (7). On other hand, CCl4 – induced hepatotoxicity cleared in severe liver damage, showed elevation of liver enzymes. The MeOH extract and n-BuOH fraction exhibited significant hepatoprotection in improvement liver enzymes, with a propensity to return to near-normal levels. So, P. crispum extracts are promising in-vivo hepatoprotective activity.

DOI

10.21608/bfsa.2022.271594

Keywords

Apiaceae, β-sitosterol, flavonoid, Hepatoprotective activity, Petroselinum crispum

Authors

First Name

Nadia

Last Name

Metwally

MiddleName

H.

Affiliation

Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt

Email

mahaelshazly@ymail.com

City

-

Orcid

-

First Name

Mortada

Last Name

El-Sayed

MiddleName

M.

Affiliation

Medicinal Chemistry Department,Theodor Bilharz Research Institute, Kornaish El-Nile-Warrak El-Hadar, Imbaba, Giza 12411, Egypt

Email

-

City

-

Orcid

-

First Name

Eman

Last Name

Morsi

MiddleName

A.

Affiliation

Medicinal Chemistry Department,Theodor Bilharz Research Institute, Kornaish El-Nile-Warrak El-Hadar, Imbaba, Giza 12411, Egypt

Email

emo_201039@yahoo.com

City

-

Orcid

0000-0002-1821-0423

First Name

Tarek

Last Name

Abou-Shousha

MiddleName

-

Affiliation

Pathology Departments,Theodor Bilharz Research Institute, Kornaish El-Nile-Warrak El-Hadar, Imbaba, Giza 12411, Egypt

Email

taboushousha@gmail.com

City

-

Orcid

-

First Name

Bassant

Last Name

Abdel-Wahab

MiddleName

S. A.

Affiliation

Medicinal Chemistry Department,Theodor Bilharz Research Institute, Kornaish El-Nile-Warrak El-Hadar, Imbaba, Giza 12411, Egypt

Email

basanttbri@gmail.com

City

-

Orcid

-

First Name

Heba

Last Name

Abdel-Hady

MiddleName

-

Affiliation

Medicinal Chemistry Department,Theodor Bilharz Research Institute, Kornaish El-Nile-Warrak El-Hadar, Imbaba, Giza 12411, Egypt

Email

h_hady10@yahoo.com

City

-

Orcid

0000-0001-8030-6904

Volume

45

Article Issue

2

Related Issue

37882

Issue Date

2022-12-01

Receive Date

2022-05-31

Publish Date

2022-12-01

Page Start

709

Page End

721

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 2_Pages 709-721.pdf

PDF . 1.2MB

Link

https://bpsa.journals.ekb.eg/article_271594.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=271594

Order

271,594

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

CHROMATOGRAPHIC ISOLATION, IDENTIFICATION AND CHARACTERIZATION OF SOME CHEMICAL CONSTITUENTS OF PETROSELINUM CRISPUM LEAVES AND EVALUATING AS HEPATOPROTECTIVE AGENTS

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Type

Article

Created At

22 Jan 2023