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271580

ESTIMATION, CHARACTERIZATION OF PHENOLICS COMPOUNDS AND IN VITRO EVALUATION OF THE ANTIOXIDANT ACTIVITY OF THYMUS FONTANESII BOISS ET REUT EXTRACTS

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Last updated: 04 Jan 2025

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Abstract

This study aimed to investigate the hydro methanolic extract and its fractions of aerial parts from Thymus fontanesii Boiss et Reut for their total phenolic and flavonoid contents, characterization of compounds in hydro methanolic extract, and antioxidant activity. The total phenolic and flavonoids were determined by Folin-Ciocalteu and aluminium chloride methods, respectively. The characterization of the crude hydromethanolic extract was done by HPLC UV-DAD, 2, 2-diphenyl-1-picrylhydrazyl (DPPH),  ferric reducing antioxidant power (FRAP), and -carotene–linoleic acid tests were used to assess antioxidant activity in vitro. The extracts' total phenolic and flavonoid concentrations ranged from 14.56–237.6 mg gallic acid equivalent (GAE)/g. extract and 1.63–2.27 mg quercetin equivalent (QE)/g extract, respectively where the ethyl acetate fraction has the highest value. The characterization of hydro methanolic extract has revealed the presence of salicylic acid, coumaric acid, rosmarinic acid synergistic acid, naringenin 7-glucoside, and luteolin 7-glucoside. The results of antioxidant tests indicated that for the antiradical test the diethyl ether fraction exhibited stronger antioxidant activity, for the FRAP test methanolic extract was more active than other fractions, at least the chloroform fraction was the stronger protector of β-carotene–linoleic acid system against lipids oxidation. Considering these results T. fontanesii Boiss et Reut aerial part can be used for developing antioxidants, that can act against various oxidative diseases.

DOI

10.21608/bfsa.2022.271580

Keywords

Thymus fontanesii, Antioxidant activity, characterization and total phenolic and flavonoid contents

Authors

First Name

Ahmed

Last Name

Nouasri

MiddleName

-

Affiliation

Laboratory of Bioactive Products and Biomass Valorization Research. ENS Kouba, BP92, Vieux Kouba Alger, Algeria

Email

a_nouasridz2001@yahoo.fr

City

-

Orcid

-

First Name

Hafitha

Last Name

Metidji

MiddleName

-

Affiliation

University Saad Dahlab, 1 BP 270 Blida (09000) faculté des sciences. Alegria.

Email

metidjihafidha@gmail.com

City

-

Orcid

-

First Name

Soumia

Last Name

Merah

MiddleName

-

Affiliation

USTHB, Faculty of Biology, Bab Ezzouar 16111, Algiers Algeria

Email

merahsoumia@yahoo.fr

City

-

Orcid

0000-0001-6651-298X

First Name

Soumeya

Last Name

Krimat

MiddleName

-

Affiliation

Laboratory of Bioactive Products and Biomass Valorization Research. ENS Kouba, BP92, Vieux Kouba Alger, Algeria

Email

krimatsoumia.2012@gmail.com

City

-

Orcid

-

First Name

Dahmane

Last Name

Dahmane

MiddleName

-

Affiliation

Laboratory of Bioactive Products and Biomass Valorization Research. ENS Kouba, BP92, Vieux Kouba Alger, Algeria

Email

dahmane12@gmail.com

City

-

Orcid

-

First Name

Aicha

Last Name

Ksouri

MiddleName

-

Affiliation

USTHB, Faculty of Biology, Bab Ezzouar 16111, Algiers Algeria.

Email

ksouri_aicha@hotmail.com

City

-

Orcid

-

Volume

45

Article Issue

2

Related Issue

37882

Issue Date

2022-12-01

Receive Date

2022-03-22

Publish Date

2022-12-01

Page Start

615

Page End

627

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 2_Pages 615-627.pdf

PDF . 1.4MB

Link

https://bpsa.journals.ekb.eg/article_271580.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=271580

Order

271,580

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

ESTIMATION, CHARACTERIZATION OF PHENOLICS COMPOUNDS AND IN VITRO EVALUATION OF THE ANTIOXIDANT ACTIVITY OF THYMUS FONTANESII BOISS ET REUT EXTRACTS

Details

Type

Article

Created At

22 Jan 2023