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239583

DETECTION OF CARBAPENEM RESISTANCE AMONG ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE IN CHEST INTENSIVE CARE UNIT AT ASSIUT UNIVERSITY HOSPITALS, EGYPT

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Last updated: 25 Dec 2024

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Abstract

This study aimed to determine antimicrobial susceptibility pattern, percentage of carbapenem resistance and presence of blaNDM1, blaVIM1, blaIMP, blaKPC genes among Escherichia coli (E.coli) and Klebsiella pneumonia (K. pneumonia) isolated from chest intensive care unit (ICU) at Assiut University Hospitals, Egypt. Antibacterial susceptibility was detected by disc diffusion method. Genotypic detection of carbapenem resistant genes (IMP, NDM, VIM, and KPC) was done by PCR.  From totally 200 patient clinical samples, 100 isolates (50%) were identified to be E.coli and K. pneumonia.  Various percentages of resistance were reported for oxacillin (80 and 60%), ciprofloxacin (46 and 52%), amikacin (66 and 86%) amoxicillin & clavulanic acid (60 and 90%) lomefloxacin (22 and 18%) gentamicin (70 and 54%) chloramphenicol (54.4 and 66.7%), imipenem (90 and 84%), meropenem (80 and 72%) for E.coli and Klebsiella. The prevalence of KPC, NDM1, IMP, VIM genes was 74, 56, 30, 26 % for E.coli and NDM1,KPC, VIM1, IMP was 66, 64, 54, 50 % For K. pneumoniae. In conclusion, carbapenemases have essential role in antibiotic resistance of E. coli and K. pneumonia.

DOI

10.21608/bfsa.2022.239583

Keywords

Carbapenem resistance, blaNDM1, blaVIM1, blaIMP, blaKPC, E.coli, K. pneumoniae

Authors

First Name

Marwa

Last Name

Mahmoud

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt.

Email

marwagad1987@gmail.com

City

-

Orcid

-

First Name

Khaled

Last Name

Hassanein

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt.

Email

khaledhassanein70@yahoo.com

City

-

Orcid

-

First Name

Tahani

Last Name

Alshammari

MiddleName

Obaid

Affiliation

Clinical laboratory, The College of Applied Medical Sciences, Aljouf University.

Email

tahanialshmri20@gmail.com

City

-

Orcid

-

First Name

Gaber

Last Name

Batiha

MiddleName

El-Saber

Affiliation

Department of Pharmacology and Therapeutics, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, AlBeheira, Egypt.

Email

-

City

-

Orcid

-

First Name

Helal

Last Name

Hetta

MiddleName

F.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71515, Egypt.

Email

helalhetta@yahoo.com

City

-

Orcid

0000-0001-8541-7304

Volume

45

Article Issue

1

Related Issue

34403

Issue Date

2022-06-01

Receive Date

2021-12-04

Publish Date

2022-06-01

Page Start

419

Page End

426

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 1_Pages 419-426.pdf

PDF . 646.7kB

Link

https://bpsa.journals.ekb.eg/article_239583.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=239583

Order

35

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

DETECTION OF CARBAPENEM RESISTANCE AMONG ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE IN CHEST INTENSIVE CARE UNIT AT ASSIUT UNIVERSITY HOSPITALS, EGYPT

Details

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Article

Created At

22 Jan 2023