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239579

SOME VIRULENCE GENES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM INFECTED VASCULAR ACCESSES IN HEMODIALYSIS PATIENTS AT ASSIUT UNIVERSITY HOSPITALS

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Last updated: 04 Jan 2025

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Abstract

We aimed in this work to detect bacterial pathogens causing infection of the vascular accesses in hemodialysis patients and demonstration of important genes responsible for virulence and biofilm formation in Staphylococcus aureus isolates namely; spa, mecA, PVL, γ-hemolysin, and icaA genes. The study was conducted from March 2018 to September 2020 and included 90 patients with infected hemodialysis accesses. Blood samples were collected for blood culture. Distal catheter end was cut and placed in brain heart infusion broth. Total bacterial isolates were 234. Samples were identified by conventional bacteriological methods. Staph. aureus was the most common isolated microorganism. Virulent genes in Staph. aureus were detected by PCR.  Fifty (96.25%) of the 52 Staph. aureus isolates were mecA gene positive. The most common spa genotype was at S3 followed by S2. Forty of 52 (76.90 %) Staph. aureus isolates were icaA positive. Only 2(3.85%) Staph. aureus isolates were gamma-hemolysin positive. All 52 isolates of Staph. aureus were negative for Panton-Valentine leukocidin gene.

DOI

10.21608/bfsa.2022.239579

Authors

First Name

Ehsan

Last Name

Hassan

MiddleName

A.

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Egypt

Email

dr_ehsan66@yahoo.com

City

-

Orcid

-

First Name

Mona

Last Name

Abdel-Rahim

MiddleName

Hussein

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Egypt

Email

mona.hussein@aun.edu.eg

City

-

Orcid

-

First Name

Thanaa

Last Name

Hassan

MiddleName

Hassan Mohamed

Affiliation

Pharmacist at Abnob Hospital, Assiut Governorate, Egypt

Email

thanaa.mbam@gmail.com

City

-

Orcid

-

First Name

Nashwa

Last Name

A. Azoz

MiddleName

Mostafa

Affiliation

Nephrology Unit, Internal Medicine Department, Assiut University Hospitals, Egypt

Email

-

City

-

Orcid

-

First Name

Mona

Last Name

Mohamed

MiddleName

Embarek

Affiliation

Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Egypt

Email

-

City

-

Orcid

-

Volume

45

Article Issue

1

Related Issue

34403

Issue Date

2022-06-01

Receive Date

2021-09-20

Publish Date

2022-06-01

Page Start

371

Page End

387

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 1_Pages 371-387.pdf

PDF . 1.3MB

Link

https://bpsa.journals.ekb.eg/article_239579.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=239579

Order

31

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

SOME VIRULENCE GENES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM INFECTED VASCULAR ACCESSES IN HEMODIALYSIS PATIENTS AT ASSIUT UNIVERSITY HOSPITALS

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Article

Created At

22 Jan 2023