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239545

PRODUCTION OF CELL WALL-HYDROLYZING ENZYMES AND GC-MS EXTRACT ANALYSIS OF BYSSOCHLAMYS LAGUNCULARIAE, A NEW RECORD ISOLATED FROM EGYPT

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Last updated: 04 Jan 2025

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Abstract

In the present work, six strains of Byssochlamys lagunculariae were isolated from alkaline wastewater of textile factory polluted with azo dyes in Alexandria, Egypt. Out of the six strains, only one was identified using the sequencing of the internal transcribed spacer gene (ITS). ITS sequence was uploaded to GenBank (MT939911) and a pure culture was maintained in the culture collection of Assiut University Mycological Centre (AUMC) as B.lagunculariae AUMC 14498. The strain shared some morphological characteristics indicating species such as the existence of smooth chlamydospores, ascospore size, and good growth on Czapek's yeast autolysate agar (CYA) at 30 °C. However, it was different from the type species by providing larger conidia and positive acid production on creatine sucrose agar (CREA) at 30 °C. Extrolite analysis of this strain was performed using gas chromatography mass spectrometry (GC-MS). The extrolite analysis extract revealed that more than fifty chemical compounds were detected, which have an important medical application. All B. lagunculariae strains tested, when grown on oat spelt xylan could utilize oat spelt xylan to produce endoglucanase, exoglucanase and xylanase as well as maize starch and pectin, respectively for amylase and pectinase production. Three strains of B. lagunculariae achieved maximum specific activity for amylase ranged from 75.65 to 114.67 IU/mg, while one of these three was superior for endoglucanase (59.1 IU/mg), exoglucanase (102.84 IU/mg), pectinase (34.33 IU/mg), and xylanase (90.21 IU/mg) production.

DOI

10.21608/bfsa.2022.239545

Keywords

Byssochlamys lagunculariae, B. lagunculariae enzymes, B. lagunculariae extrolite, : Byssochlamys isolation

Authors

First Name

Osama

Last Name

Al-Bedak

MiddleName

A.

Affiliation

Assiut University Mycological Centre, Assiut University, Assiut 71511, Egypt

Email

osamaalbedak@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Abdel-Sater

MiddleName

A.

Affiliation

Department of Botany and Microbiology, Faculty of Science, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Hagar

Last Name

Galal

MiddleName

M.K.

Affiliation

Department of Botany and Microbiology, Faculty of Science, New Valley University, El-Kharga 72511, Egypt

Email

-

City

-

Orcid

-

First Name

Sedky

Last Name

Hassan

MiddleName

H.A.

Affiliation

Department of Botany and Microbiology, Faculty of Science, New Valley University, El-Kharga 72511, Egypt/ Department of Biology, College of Science, Sultan Qaboos University, Muscat 123, Oman

Email

s.hassan@squ.edu.om

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-

Orcid

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Volume

45

Article Issue

1

Related Issue

34403

Issue Date

2022-06-01

Receive Date

2021-08-24

Publish Date

2022-06-01

Page Start

359

Page End

369

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 1_Pages 359-369.pdf

PDF . 1.1MB

Link

https://bpsa.journals.ekb.eg/article_239545.html

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https://bpsa.journals.ekb.eg/service?article_code=239545

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

PRODUCTION OF CELL WALL-HYDROLYZING ENZYMES AND GC-MS EXTRACT ANALYSIS OF BYSSOCHLAMYS LAGUNCULARIAE, A NEW RECORD ISOLATED FROM EGYPT

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Article

Created At

22 Jan 2023