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239504

EFFECT OF COPPER SULPHATE POLLUTION AND ITS ANTIDOTE PENICILLAMINE ON SERUM AND BRAIN TISSUES MARKERS OF ALBINO RATS

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Last updated: 25 Dec 2024

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Abstract

Background: Copper is an essential trace element and is required for many metabolic functions. Aim of work: The present study was designed to study the negative impact of excess copper sulphate on the central neural function of albino rats and studying how its antidote d-penicillamine play role in improving its side effects. Material and methods: Seventy albino rats were divided into seven equal groups each containing 10 rats (G1:control received distilled water) ; (G2:0.1 LD50 of CuSO4) ; (G3:0.2 LD50 of CuSO4) ; (G4:0.4 LD50 of CuSO4); (G5:0.1 LD50 of CuSO4 +100mg/kg/day of penicillamine) ; (G6:LD50 of CuSO4.+100 mg/kg/day of penicillamine) and (G7:LD50 of CuSO4+ 100 mg /kg/day of penicillamine) for 30 days and at the end of the experiment all rats were sacrificed and blood samples and brain tissues were collected for biochemical assaying of fasting blood glucose (FBG), serum Cu level, serum tyrosinase activity, oxidative stress marker malondialdehyde (MDA) and total antioxidant activity (TAC). also, DNA determination of relative gene expression of cerebral adenosine monophosphate-activated protein kinase (AmpK), protein kinase (AKT), phosphatidylinositol-3-kinase (PI3K), cytochrome c oxidase (Cyto co ), and glucose -6- phosphate dehydrogenase (G6PD). Results: The results showed that administration of copper sulphate with different levels induced a significant increase in fasting blood glucose level, lipid peroxidation marker MDA, serum copper level, and serum tyrosinase activity, and a significant decrease in TAC. Moreover, copper sulphate administration elicited a significant downregulation ( AmpK, AKT, PI3K, Cytochrome c oxidase, G6PD).it could be approved that penicillamine could abolish the negative impact of copper sulphate on neural tissues and serum enzymes. Conclusion: D-penicillamine can reduce neurotoxicity and oxidative stress caused by copper pollution. Recommendations: Exposure to the pollution of copper must be controlled. Search for sensitive blood and neural markers for early detection of neurological disorders and further studies are needed to use its antidote d-penicillamine in the treatment of copper-induced pollution.

DOI

10.21608/bfsa.2022.239504

Keywords

D- Penicillamine, Copper sulphate pollution, albino rats, Gene expression

Authors

First Name

Medhat

Last Name

Fawzy

MiddleName

M.

Affiliation

Biochemistry Department Faculty of Veterinary Medicine, Zagazig University, Egypt

Email

medhatfawzy_vet@yahoo.com

City

-

Orcid

-

First Name

Sara

Last Name

Ahmed

MiddleName

M.

Affiliation

Bachelor of Pharmacy, Zagazig University

Email

-

City

-

Orcid

-

First Name

Tarek

Last Name

Khamis

MiddleName

-

Affiliation

3Department of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig, Egypt/ 4Laboratory of Biotechnology, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig, Egypt

Email

tarekkhamis13@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Hamed

MiddleName

-

Affiliation

Department of Animal Physiology and Biochemistry, Faculty of Veterinary Medicine, Badr University in Cairo (BUC), Badr City, Cairo, Egypt/ Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig, Egypt

Email

vetahmedhamed@yahoo.com

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-

Orcid

-

First Name

Doaa

Last Name

Abdel-Fattah

MiddleName

M.

Affiliation

Biochemistry Department Faculty of Veterinary Medicine, Zagazig University, Egypt

Email

drdoaa30@yahoo.com

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-

Orcid

-

Volume

45

Article Issue

1

Related Issue

34403

Issue Date

2022-06-01

Receive Date

2021-09-12

Publish Date

2022-06-01

Page Start

327

Page End

337

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 1_Pages 327-337.pdf

PDF . 781.3kB

Link

https://bpsa.journals.ekb.eg/article_239504.html

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https://bpsa.journals.ekb.eg/service?article_code=239504

Order

27

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

EFFECT OF COPPER SULPHATE POLLUTION AND ITS ANTIDOTE PENICILLAMINE ON SERUM AND BRAIN TISSUES MARKERS OF ALBINO RATS

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Article

Created At

22 Jan 2023