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239458

A VALIDATED HPLC METHOD FOR SEPARATION AND DETERMINATION ASPARTAME AND ACESULFAME-K IN FOOD PRODUCTS

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Last updated: 04 Jan 2025

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Abstract

A pragmatic, economical, precise, straightforward to execute, and selective method for separation and determination of Aspartame and Acesulfame-k concentrations in non-alcoholic beverages and soft drinks.The separation by HPLC was implemented using a Welchrom C18 column (4.6 x 250 mm, 5µm) at 30°C temperature and a SHIMADZU UV-photo diode array detector at 217, 226 nm for Aspartame and Acesulfame-k respectively.The mobile phase composition consisted of potassium dihydrogen phosphate pH= 4.5 and acetonitrile (80:20 v/v) with a flow rate of 1mL/min and an injection volume of 10 µL.The retention time of Acesulfame-k and Aspartame were 2.94, 6.51 respectively. The analysis time was less than 10 minutes.
This method demonstrated appropriate results of linearity, precision, and recovery. It was applied efficiently to analyze Aspartame and Acesulfame-k.The calibration curve was linear with R2 >0.999. The precision values of percentage relative standard deviation were less than 2 %RSD

DOI

10.21608/bfsa.2022.239458

Keywords

Analytical chemistry, Chromatography, HPLC, Aspartame, acesulfame-k

Authors

First Name

Nermeen

Last Name

Barakat

MiddleName

Mahmoud

Affiliation

Department of Food Engineering Technology, Faculty of Technical Engineering, University of Aleppo, Syria

Email

nermeenmahmoudba@gmail.com

City

-

Orcid

-

First Name

Mohammed

Last Name

Al-Azem

MiddleName

-

Affiliation

Department of Food Engineering Technology, Faculty of Technical Engineering, University of Aleppo, Syria

Email

mohmadazem@yahoo.com

City

-

Orcid

-

First Name

Nazira

Last Name

Sarkis

MiddleName

-

Affiliation

Department of Analytical and Food Chemistry, Faculty of Pharmacy, University of Aleppo, Syria

Email

nazirasarkis@gmail.com

City

-

Orcid

-

First Name

Saleh

Last Name

Trefi

MiddleName

-

Affiliation

Department of Pharmaceutical Chemistry and Quality Control, Faculty of Pharmacy, University of Aleppo, Syria

Email

trefiquality@gmail.com

City

-

Orcid

-

Volume

45

Article Issue

1

Related Issue

34403

Issue Date

2022-06-01

Receive Date

2022-02-05

Publish Date

2022-06-01

Page Start

269

Page End

274

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 1_Pages 269-274.pdf

PDF . 1.1MB

Link

https://bpsa.journals.ekb.eg/article_239458.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=239458

Order

21

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

A VALIDATED HPLC METHOD FOR SEPARATION AND DETERMINATION ASPARTAME AND ACESULFAME-K IN FOOD PRODUCTS

Details

Type

Article

Created At

22 Jan 2023