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239374

EXPLORATION OF COMPONENTS CONTRIBUTING TO POTENT CYTOTOXICITY OF GARDENIA THUNBERGIA L. F. AGAINST HUMAN LEUKEMIA AND HEPATOMA

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Last updated: 04 Jan 2025

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Abstract

Promising cytotoxic effects of several Gardenia species (Rubiaceae) have been established by many studies. The current study evaluated MTT-based cytotoxic activities of the crude extract from Gardenia thunbergia L. f. aerial parts and four fractions thereof, including n-hexane, dichloromethane (DCM), ethyl acetate, and aqueous, against human leukemia (HL-60) and hepatoma (HepG2) cell lines, as well as the normal (WI38) cell line. Both non-polar fractions, n-hexane and dichloromethane, showed tumor-selective toxicities against both tested cancerous cell lines. These results sparked our interest in chemically characterising these bioactive fractions to reveal their cytotoxic components. The composition of n-hexane-soluble fraction was investigated via GC-MS analysis, while column chromatographic separation was used to isolate the components of DCM-soluble fraction. These isolated phytochemicals were identified via spectroscopic analyses. Besides, the chemotaxonomic value of the detected phytochemicals and their reported cytotoxic profiles were discussed.

DOI

10.21608/bfsa.2022.239374

Keywords

Gardenia thunbergia, Rubiaceae, Cytotoxicity, Human leukemia, HepG2 cells, chemotaxonomy

Authors

First Name

Shaymaa

Last Name

Mohamed

MiddleName

M.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71515, Egypt

Email

shaymaa.makram@aun.edu.eg

City

-

Orcid

0000-0001-7768-0890

First Name

Samir

Last Name

Ross

MiddleName

-

Affiliation

National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy, The University of Mississippi, University, Mississippi 38677, USA , Department of BioMolecular Sciences, Division of Pharmacognosy, School of Pharmacy, University of Mississippi, University, Mississippi 38677, USA

Email

sross@olemiss.edu

City

-

Orcid

-

First Name

Nesma

Last Name

Mohamed

MiddleName

M.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71515, Egypt.

Email

nesma.mohamed269@pharm.aun.edu.eg

City

-

Orcid

0000-0001-8357-6942

Volume

45

Article Issue

1

Related Issue

34403

Issue Date

2022-06-01

Receive Date

2022-01-05

Publish Date

2022-06-01

Page Start

153

Page End

162

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 45_Issue 1_Pages 153-162.pdf

PDF . 924.6kB

Link

https://bpsa.journals.ekb.eg/article_239374.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=239374

Order

13

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

EXPLORATION OF COMPONENTS CONTRIBUTING TO POTENT CYTOTOXICITY OF GARDENIA THUNBERGIA L. F. AGAINST HUMAN LEUKEMIA AND HEPATOMA

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Article

Created At

22 Jan 2023