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ANTIDIABETIC ACTIVITY, ALPHA-AMYLASE AND ALPHA- GLUCOSIDASE INHIBITORY EFFECT OF PASTINACA SATIVA EXTRACT

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Last updated: 04 Jan 2025

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Abstract

Pastinaca sativa belonging to the family Apiaceae, is a tropical tree used in many
countries as a herbal drug for the care of diabetic patients. However, the methodical rationale
for this medical use is very limited. The aim of this analysis was to examine the antidiabetic
activity of the Pastinaca sativa methanolic extract and the possible mechanisms underlying that
activity. The extract hypoglycemic behavior was investigated in typical diabetic rats induced
with alloxan. Finally, an effect of the Pastinaca sativa extract (Crude extract of Pastinaca
sativa-CEPS) on the activity of α-amylase and α-glucosidase was examined in vitro. CEPS
showed IC50 equal to 91.69 ± 1.5 μg/mL for α-amylase enzyme inhibition activity. Normal
acarbose (control) demonstrated IC50 equal to 83.25 ± 1.28 μg/mL. CEPS displayed IC50
values of 88.05 ± 1.25 μg/mL for α-glucosidase enzyme. Under similar laboratory conditions,
acarbose displayed an IC50 value of 51.00 ± 1.23 μg/mL. CEPS exhibited IC50 less than
100μg/mL would be considered as healthy. The extract at 400 mg/kg greatly decreased the
region under the blood glucose level curve in a typical rats test for oral glucose tolerance. A
single dose of the extract decreased significantly in the alloxan-induced diabetic model, similar
to glibenclamide (standard), from 208.33 mg/dl to 106.38 mg/dl at 200 mg/kg CEPS and from
209.82 mg/dl to 111.65 mg/dl at 400 mg/kg CEPS. These findings were comparable with 0.5
mg/kg of glibenclamide indicating a substantial decrease from 205.55 mg/dl on 7th day to
84.88 mg/dl. The Pastinaca sativa methanolic extract possesses strong antidiabetic activity in
vivo. Besides, the extract has also been shown to have a significant inhibitory activity of α-
amylase and α-glucosidase, which might lead to its anti-hyperglycemic function when used in
diabetic patients.

DOI

10.21608/bfsa.2021.207157

Authors

First Name

Nagaraju

Last Name

J

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Affiliation

K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, India 522 502 , Department of Pharmacology, Balaji Institute of Pharmaceutical Sciences, Laknepally, Narsampet, Warangal, Telangana, India 506331

Email

nagarajupharma@gmail.com

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First Name

Rajasekhar

Last Name

A

MiddleName

Reddy

Affiliation

K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, India 522 502

Email

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-

Orcid

-

First Name

Subhakar

Last Name

Raju R

MiddleName

-

Affiliation

K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, India 522 502

Email

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City

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Orcid

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First Name

Koteswara

Last Name

Rao GSN

MiddleName

-

Affiliation

K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, India 522 502

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-

Orcid

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First Name

Prasanth

Last Name

DSNBK

MiddleName

-

Affiliation

K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, India 522 502

Email

-

City

-

Orcid

-

First Name

Chakravarthi

Last Name

G

MiddleName

-

Affiliation

K L College of Pharmacy, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Guntur, Andhra Pradesh, India 522 502

Email

-

City

-

Orcid

-

Volume

44

Article Issue

2

Related Issue

29160

Issue Date

2021-12-01

Receive Date

2021-07-13

Publish Date

2021-12-01

Page Start

387

Page End

395

Print ISSN

1110-0052

Online ISSN

3009-7703

Link

https://bpsa.journals.ekb.eg/article_207157.html

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10

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

ANTIDIABETIC ACTIVITY, ALPHA-AMYLASE AND ALPHA- GLUCOSIDASE INHIBITORY EFFECT OF PASTINACA SATIVA EXTRACT

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Article

Created At

22 Jan 2023