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174149

CHARACTERIZATION, IN-VITRO EVALUATION OF PROBIOTIC POTENTIAL AND ANTAGONISTIC ACTIVITY OF SELECTED LACTIC ACID BACTERIA STRAINS ISOLATED FROM NATURAL ORIGIN AGAINST SOME HUMAN PA

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Last updated: 25 Dec 2024

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Abstract

Probiotic bacteria have recently become popular for their health-related beneficial effects. In this study, 19 lactic acid bacteria were isolated from breast milk, dairy products, infant stool, vaginal swabs, fermented beverages, and fermented grapes. These isolates were evaluated for their tolerance to gastrointestinal conditions, hydrophobicity, auto-aggregation, and antimicrobial activity. Five isolates, belonging to Pediococcus spp. and Lactobacillus spp., showed potential probiotic features. These isolates were tested for the presence of virulence enzymes and for susceptibility to various antibiotics. The isolates were found to be non-hemolytic and lacked gelatinase and deoxyribonuclease enzymes, however, some antibiotic resistance genes were detected. To ensure the safety of the used probiotics, cell-free supernatants (CFS) of the promising candidates were prepared and its antimicrobial activity before and after neutralization was assessed against standard strains of Candida albicans, Gram-positive, and Gram-negative bacteria. The neutralization of the CFS significantly diminished the antibacterial and antifungal activities of probiotics. Using the time-kill assay, the combination of CFS of Lactiplantibacillus plantarum with gentamicin and ceftazidime was tested against Staphylococcus aureus and Escherichia coli clinical isolates. A significant reduction in log number of survivors was obtained with gentamicin against E. coli and with ceftazidime against both isolates. The anti-biofilm ability of the CFS of the selected probiotics was tested as well. Neutralized CFS caused 26-52% inhibition of S. aureus isolate biofilm formation compared to the control. In conclusion, the five selected isolates could be considered promising probiotic candidates that can be used as biotherapeutics in case of bacterial infection.

DOI

10.21608/bfsa.2021.174149

Authors

First Name

Azza

Last Name

Zakaria

MiddleName

S.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt

Email

azza.hanafy@alexu.edu.eg

City

-

Orcid

0000-0003-3772-1381

First Name

Mervat

Last Name

Kassem

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt

Email

mervat.kassem@alexu.edu.eg

City

-

Orcid

-

First Name

Mona

Last Name

El Far

MiddleName

S.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt

Email

s-mona.elfar@alexu.edu.eg

City

-

Orcid

-

First Name

Eva

Last Name

Edward

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt

Email

-

City

-

Orcid

-

Volume

44

Article Issue

1

Related Issue

25283

Issue Date

2021-06-01

Receive Date

2021-03-02

Publish Date

2021-06-01

Page Start

225

Page End

241

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 44_Issue 1_Pages 225-241.pdf

PDF . 1.8MB

Link

https://bpsa.journals.ekb.eg/article_174149.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=174149

Order

22

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

CHARACTERIZATION, IN-VITRO EVALUATION OF PROBIOTIC POTENTIAL AND ANTAGONISTIC ACTIVITY OF SELECTED LACTIC ACID BACTERIA STRAINS ISOLATED FROM NATURAL ORIGIN AGAINST SOME HUMAN PATHOGENS

Details

Type

Article

Created At

22 Jan 2023