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174147

VIRULENCE PROFILE OF HELICOBACTER PYLORI DETECTED IN GASTRIC BIOPSIES OF PATIENTS UNDERGOING ENDOSCOPY IN UPPER EGYPT

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Last updated: 25 Dec 2024

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Abstract

Background: Helicobacter pylori (H. pylori) is a common cause of gastric ulcers and is a risk factor for gastric carcinoma. Little data are available about the characters of H. pylori causing infections in patients from Upper Egypt. Therefore, the aim of this study was to investigate the frequency of H. pylori infection in Egyptian patients undergoing endoscopy and complaining of persistent upper gastrointestinal symptoms directly in biopsy specimens using PCR technique and to study the associated virulence-related genes in H. pylori positive samples. Materials and Methods: One hundred and twenty gastric biopsy specimens were collected from Egyptian patients admitted to Assiut University Hospital and complaining of persistent upper gastrointestinal symptoms. H. pylori DNA was extracted for molecular identification by PCR. Positive samples were further analyzed to investigate the presence of different virulence-related genes and gene combinations. Results: H. pylori was detected in 92/120 (76.6%) of collected biopsies. All strains carried the vacA s1 subtype. The prevalence of the virulence genes cagA, cagE, iceA1, iceA2 and oipA were 79 (84.8%), 41(44.5%), 69 (75%), 44 (47.8%), and 55(59.7%) of the H. pylori positive samples, respectively. Genetic analysis showed that H. pylori were grouped into 29 different genotype combinations. The s1/m1/i1/d1/c1/cagA/iceA1/oipA genotype was the most predominant. Conclusion: Our results show a high frequency of H. pylori infections among Egyptian patients with gastrointestinal symptoms. The s1/m1/i1 vacA hybrid is the most prevalent subtype. The distribution of the different virulence-related genes shows alarming rates of cagA, cagE, iceA1, iceA2 and oipA. Careful monitory of H. pylori infections and investigation of their genetic characters should be carried out to control the spread of the virulent strains.

DOI

10.21608/bfsa.2021.174147

Authors

First Name

Sahar

Last Name

Mandour

MiddleName

A.

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Deraya University, Minia 61111, Egypt

Email

saharmandour2012@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Abdelmohsen

MiddleName

Shawkat

Affiliation

Department of Tropical Medicine and Gastroenterology, Faculty of Medicine Assiut University, Assiut, Egypt

Email

shawkatamhed84@aun.edu.eg

City

-

Orcid

-

First Name

Mohammed

Last Name

Medhat

MiddleName

A.

Affiliation

Department of Tropical Medicine and Gastroenterology, Faculty of Medicine Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

First Name

Doaa

Last Name

Abdeltawab

MiddleName

-

Affiliation

Department of Tropical Medicine and Gastroenterology, Faculty of Medicine Assiut University, Assiut, Egypt

Email

doaaabdeltawab@gmail.com

City

-

Orcid

-

First Name

Ameer

Last Name

Elfarash

MiddleName

-

Affiliation

Department of Genetics, Faculty of Agriculture, Assiut University, Assiut, Egypt

Email

aelfarash@aun.edu.eg

City

-

Orcid

-

First Name

Abdulrahman

Last Name

Ahmed

MiddleName

K.

Affiliation

Medical Student, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

abdulrahmankhalifan7100@gmail.com

City

-

Orcid

0000-0002-5134-1089

First Name

Ahmed

Last Name

Galal

MiddleName

M.

Affiliation

Postgraduate Student, Department of Surgical Oncology, Sohag Cancer Center, Sohag, Egypt

Email

ahmed_g999@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

El-Mokhtar

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

-

City

-

Orcid

-

Volume

44

Article Issue

1

Related Issue

25283

Issue Date

2021-06-01

Receive Date

2021-03-05

Publish Date

2021-06-01

Page Start

213

Page End

224

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 44_Issue 1_Pages 213-224.pdf

PDF . 509.1kB

Link

https://bpsa.journals.ekb.eg/article_174147.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=174147

Order

21

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

VIRULENCE PROFILE OF HELICOBACTER PYLORI DETECTED IN GASTRIC BIOPSIES OF PATIENTS UNDERGOING ENDOSCOPY IN UPPER EGYPT

Details

Type

Article

Created At

22 Jan 2023