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174144

PLASMA EPSTEIN-BARR VIRUS (EBV) DNA AS A BIOMARKER FOR DIAGNOSIS OF SYRIAN EBV-POSITIVE BURKITT’S LYMPHOMA

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Last updated: 04 Jan 2025

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Abstract

Epstein-Barr virus - positive Burkitt's Lymphoma is defined as the presence of Epstein-Barr virus (EBV) in tumor cells, the standard way to detect (EBV) in Burkitt's Lymphoma is in-situ hybridization (ISH) of EBV-encoded small RNA (EBERs) in tumor cells. The present study aimed to evaluate plasma Epstein-Barr virus (EBV) DNA as a noninvasive biomarker for diagnosis and prognosis of EBV-positive Burkitt's lymphoma. The study included 40 newly diagnosed patients with Burkitt's lymphoma, ranging in age from 4 to 60 years, and 55 sex and age-matched controls. Forty formalin-fixed paraffin-embedded blocks of Burkitt's lymphoma tissue samples were used to investigate the EBV by in-situ hybridization detection of the EBERs. Plasma EBV DNA was quantified by real-time quantitative polymerase chain reaction (PCR) for all Burkitt lymphoma patients prior to therapy and for control. The results showed that (22/40, 55%) of Burkitt lymphoma were positive for histological EBER, whereas plasma EBV DNA was detectable (range from 1.2×104 to 4.7×106 copies/mL) in all EBV-positive Burkitt lymphoma samples (22/22). EBV DNA was undetectable in all cases of EBV-negative Burkitt lymphomas (18/18) and all healthy control (55/55). It is worth mentioning that our results demonstrated that the EBV DNA load was significantly high in the EBV-positive BL patients suffering poor prognostic state. In conclusion: Plasma EBV-DNA can be used as a noninvasive biomarker for diagnosis and prognosis of EBV-positive Burkitt's lymphoma.

DOI

10.21608/bfsa.2021.174144

Authors

First Name

Rana

Last Name

Habeeb

MiddleName

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Affiliation

Department of Biochemistry and Microbiology, Faculty of Pharmacy, Damascus University, Damascus, Syria

Email

ranahabeeb79@gmail.com

City

-

Orcid

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First Name

Lina

Last Name

Al Hafar

MiddleName

-

Affiliation

Department of Pathology, Faculty of Medicine, Damascus University, Damascus, Syria

Email

-

City

-

Orcid

-

First Name

Fawza

Last Name

Monem

MiddleName

-

Affiliation

Department of Biochemistry and Microbiology, Faculty of Pharmacy, Damascus University, Damascus, Syria & Department of Clinical Laboratories, Al-Assad Hospital, Damascus University, Damascus, Syria

Email

fawzamonem@hotmail.com

City

-

Orcid

0000-0002-1695-0524

Volume

44

Article Issue

1

Related Issue

25283

Issue Date

2021-06-01

Receive Date

2020-12-16

Publish Date

2021-06-01

Page Start

185

Page End

194

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 44_Issue 1_Pages 185-194.pdf

PDF . 488.0kB

Link

https://bpsa.journals.ekb.eg/article_174144.html

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https://bpsa.journals.ekb.eg/service?article_code=174144

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

PLASMA EPSTEIN-BARR VIRUS (EBV) DNA AS A BIOMARKER FOR DIAGNOSIS OF SYRIAN EBV-POSITIVE BURKITT’S LYMPHOMA

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Article

Created At

22 Jan 2023