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174141

IDENTIFICATION AND CHARACTERIZATION OF METALLO--LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES IN AL-AZHER UNIVERSITY HOSPITAL, ASSIUT

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Last updated: 25 Dec 2024

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Abstract

Purpose: Study aimed to determine the frequency of nosocomial infections caused by P. aeruginosa and its distribution among different intensive care units (ICUs), to determine the antibiotic resistance pattern, and to determine the prevalence of Metallo-b-lactamase (MbL) among isolates by phenotypic methods. Methods: A total of 74 P. aeruginosa isolates were identified from different clinical specimens in AL-Azher University Hospital. The antimicrobial susceptibility was tested by disk diffusion (Kirby-Bauer) method and phenotypic screening for MbLs was performed using Combined Disk Test (CDT) and double-disk synergy test (DDST). Results: The frequency of P. aeruginosa isolates from clinical specimens was 18% (74/412). The highest sensitivity was to imipenem 38 isolates (51.3%). The antibiotic sensitivity was in descending manner to meropenem (48.6%) > levofloxacin (28.4%) > ciprofloxacin and ceftazidime (21.6%), while the highest resistance rates were to carbenicillin 70 isolates (94.6%) then gentamicin (70.3%). Out of 74 P. aeruginosa isolates, 51/74 (69%) strains were multidrug resistant (MDR), based on the CDT results 26/74 (35.1%) isolates and by DDST 23/74 (31.1%) isolates were confirmed to be MbLs producers. Conclusion: There is a growing risk for isolation of MDR P. aeruginosa among the MBL-producer isolates suggests the need for continuous assessment of antimicrobial susceptibility and surveillance of antibiotic prescription. In addition, infection control measures are needed to prevent further dissemination of these organisms.

DOI

10.21608/bfsa.2021.174141

Authors

First Name

Rehab

Last Name

Mousa

MiddleName

-

Affiliation

Pharmacist at Al-Azher University Hospital, Assiut, Egypt and Deparmtent of Biotechnology and Life Sciences, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt

Email

-

City

-

Orcid

-

First Name

Magdy

Last Name

El-Kady

MiddleName

F.

Affiliation

Department of Poultry Diseases, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt

Email

mfelkady@yahoo.com

City

-

Orcid

-

First Name

Asmaa

Last Name

Hussein

MiddleName

A. A.

Affiliation

Department of Zoonoses, Faculty of Veterinary Medicine, and Dean of Molecular Biology Researches & Studies Institute, Assiut University, Assiut, Egypt

Email

asmaa@yahoo.com

City

-

Orcid

-

First Name

Amr

Last Name

Ahmed

MiddleName

ُE.

Affiliation

Deparmtent of Biotechnology and Life Sciences, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt

Email

amreahmed@hotmail.com

City

-

Orcid

-

First Name

Ahmed

Last Name

El-Gendy

MiddleName

Osama

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt

Email

dr.ahmed_micro@yahoo.com

City

-

Orcid

-

Volume

44

Article Issue

1

Related Issue

25283

Issue Date

2021-06-01

Receive Date

2020-10-11

Publish Date

2021-06-01

Page Start

161

Page End

172

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 44_Issue 1_Pages 161-172.pdf

PDF . 712.2kB

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https://bpsa.journals.ekb.eg/article_174141.html

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https://bpsa.journals.ekb.eg/service?article_code=174141

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16

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

IDENTIFICATION AND CHARACTERIZATION OF METALLO--LACTAMASES PRODUCING PSEUDOMONAS AERUGINOSA CLINICAL ISOLATES IN AL-AZHER UNIVERSITY HOSPITAL, ASSIUT

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Article

Created At

22 Jan 2023