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174136

HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM

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Last updated: 04 Jan 2025

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Abstract

Fetal bovine serum (FBS) is frequently used as a growth factor and as a source of proteins in culture media, but it may contain pathogens.In this study, we aimed to evaluate the possibility of using human platelet lysate (HPL) as a substitute for FBSin bone marrow karyotyping medium. The study included 30 samples of bone marrow aspiration from patients attending the Aleppo University Hospital. Our results showed that the concentration of IGF-1 and GH in HPL was (117 ng/ml) and (2.6 mIU/L) respectively.We also determined the mitotic index (MI), and the results showed that MI values were higher when using the medium supplied with HPLcompared to medium supplied with FBS.Statistical study also showed that there were significant differences (p= 0.001) in MI values when comparing the two media. Our results suggest that HPLcan be used as a substitute for FBSin bone marrow karyotyping medium.

DOI

10.21608/bfsa.2021.174136

Authors

First Name

Abdulkader

Last Name

Mima

MiddleName

-

Affiliation

Department of Biotechnology Engineering, Faculty of Technology, Aleppo University, Aleppo, Syria

Email

ph.d.abdulkader.memeh@gmail.com

City

-

Orcid

0000-0003-3955-2913

First Name

Abdel Galil

Last Name

Ghriwati

MiddleName

-

Affiliation

Department of Biotechnology Engineering, Faculty of Technology, Aleppo University, Aleppo, Syria

Email

-

City

-

Orcid

-

First Name

Khalid

Last Name

Khanji

MiddleName

-

Affiliation

Department of Pediatrics, Faculty of Medicine, Aleppo University, Aleppo, Syria

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-

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-

Orcid

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Volume

44

Article Issue

1

Related Issue

25283

Issue Date

2021-06-01

Receive Date

2020-10-19

Publish Date

2021-06-01

Page Start

111

Page End

118

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 44_Issue 1_Pages 111-118.pdf

PDF . 530.0kB

Link

https://bpsa.journals.ekb.eg/article_174136.html

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https://bpsa.journals.ekb.eg/service?article_code=174136

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11

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM

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Article

Created At

22 Jan 2023