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104595

COMPARISON OF TWO METHODS FOR MEASURING PYRETHROID HYDROLYZING ESTERASES: EXTRACTION SELECTIVITY AND RADIOLABELED IDENTITY

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Last updated: 04 Jan 2025

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Abstract

Two published methods are used widely for measuring pyrethxoid hydrolyzing esterases. These two methods are based on measuring either the acid production (Jao and Casida, 1974) or the residual unhydrolyzed ester (Suzuki and Miyamoto, 1978). These methods were compared using (IRS, tirans), permethrin, labeled at the carbonyl carbon of the acid moiety, and rat liver microsomes. No consistent results were obtained and the enzyme activity determined by the first method was lower than that of the second one. The selectivity of the extract-ion procedure in method I and the identity of the labeled product were examined by TLC chromatography. Chloroform extract in acid production method contained more than 30% of the total acid released, thereby, introducing a large error in quantitation. Alkalinization of the reaction mixture prior to chloroform extraction using 1 ml buffer, pH 10, changed the extraction selectivity of acid permethrin (DCVA) in favour of the aqueous phase and consequently increased the accuracy of this method. No chemical hydrolysis of (IRS, trans) permethrin could be detected under conditions of pH 10 over 60 minute period.

DOI

10.21608/bfsa.1981.104595

Authors

First Name

Yehia

Last Name

Abdel-Aal

MiddleName

A. I.

Affiliation

Department of Plant Protection, College of Agriculture, Assiut University, Assiut, Egypt

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Orcid

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First Name

David

Last Name

Soderlund

MiddleName

M.

Affiliation

NYSAES Department of Entomology, Geneva, N.Y. 14456

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Volume

4

Article Issue

1

Related Issue

15861

Issue Date

1981-12-01

Publish Date

1981-12-31

Page Start

151

Page End

162

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 4_Issue 1_Pages 151-162.pdf

PDF . 449.4kB

Link

https://bpsa.journals.ekb.eg/article_104595.html

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https://bpsa.journals.ekb.eg/service?article_code=104595

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11

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

COMPARISON OF TWO METHODS FOR MEASURING PYRETHROID HYDROLYZING ESTERASES: EXTRACTION SELECTIVITY AND RADIOLABELED IDENTITY

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Article

Created At

22 Jan 2023