Eudragit polymeric films were investigated as potential drug delivery systems for the controlled release of chloramphenicol sodium succinate (CSS)​.  CSS at 10​% wt.​/wt. in polymeric film of Eudragit RL100, plasticized with glycerol triacetate (GTA)​, glycerol tributyrate (GTB)​, propylene glycol (PG) or di-​Me phthalate (DMP) was prepd.  Increasing drug release rate from Eudragit RL100 films due to plasticizers could be arranged as follows: GTA > PG > DMP > GTB.  The release of CSS in polymeric film of Eudragit RL100 was studied as a function of initial drug concn. and GTA content.  The release rate const. of the drug was found to be proportional to drug concn. and GTA content in the film.  CSS films composed of different ratios of Eudragit RS100 and Eudragit RL100 were prepd.  The results show that CSS release from film contg. Eudragit RS100​/RL100 at a ratio of 2:8 was higher than those of the other polymer ratios.  The effect of addn. of different concns. of hydroxypropyl methylcellulose (HPMC) to Eudragit RS100 films on the release rate of CSS was also studied.  The best concn. of HPMC on increasing the release rate of the drug was found to be 10​% wt.​/wt.  The results indicate that drug release from matrix follows a diffusion-​controlled model.  The min. inhibitory concn. (MIC) of CSS for Staphylococcus aureus, Bacillus cereus, E. coli and Candida albicans was investigated.  Results indicated that MIC of CSS was ranged from 7 to 12 μg​/mL.  The inhibition zone diams. of CSS film composed of Eudragit RS100​/RL100 (2:8) were higher than that of CSS gauze dressing.  In-​vitro antimicrobial activity of CSS was enhanced in Eudragit RS100​/RL100 film plasticized with 20​% GTA in presence of 10​% Tween 80.