Formulation of the antibiotic, ciprofloxacin hydrochloride suppositories was carried out using different semisynthetic lipophilic bases as suppocire AM, suppocire CM, Novata B, Novata BD, Witepsol W35, and natural lipophilic base as Cocoa butter.  The in-​vitro release study of 100 mg ciprofloxacin hydrochloride suppository from these bases was performed in distd. water at 37° using thermostatically controlled mech. shaker water bath and the drug was measured at 278 nm.  The results obtained revealed that suppocire AM gave the highest release rate followed by Novata BD, Novata B, Witepsol W35, Cocoa butter and Suppocire CM resp.  The bioavailability of ciprofloxacin hydrochloride from suppositories contg. 100 mg of the drug after rectal administration in rabbits (1.5-​2 kg)​, was carried out.  HPLC procedures were adopted for the detn. of drug in plasma.  The results obtained revealed that it was in agreement with the in-​vitro data.  The results showed that the drug was regularly absorbed and provided AUC ranging between 10.3 to 20.23 μ.hr.​/mL.  Other pharmacokinetic parameters as C_max, T_max, K_a, K_e,