Liposomes were prepd. by extrusion and freeze-​thawing method using carboxyfluorescein as marker.  The effect of the no. of extrusion, Freeze-​thawing cycles on lipid content, liposome size and encapsulation efficiency was investigated.  The results revealed that, there was a direct relationship between the membrane pore size and particle size of liposomes.  However, there was an inverse relation between the no. of extrusion cycles and size of liposomes.  In the meantime, increasing the no. of extrusion cycles produced homogeneous liposomes.  Also an inverse relation was found between the no. of extrusion cycles and encapsulation efficiency of the marker.  Freeze-​thawing process offered high encapsulation efficiency of carboxyfluorescein inside liposomes.