Seven flavonoids were isolated viz., 3,6,7,4'-tetramethoxy-5,3'-dihydroxy flavonol, 5,7,4'-
trihydroxy-6-methoxy flavone (hispedulin), apigenin, 5,7,3',4'-tetrahydroxy-6-methoxy flavone,
luteolin, luteolin-7-O-glucoside, acacetin-7-O-rutinoside, in addition to  -sitosterol and  -
sitosterol glucoside from Onopordon heteracanthum. The structures of these compounds were
established through the chemical and spectral studies. All the isolated compounds were
reported for the first time from the titled plant, while the compounds 3,6,7,4'-tetramethoxy-5,3'-
dihydroxy flavonol, 5,7,3'4'-tetrahydroxy-6-methoxy flavone and acacetin-7-O-rutinoside were
isolated for the first time from the genus Onopordon.