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ENTEROCOCCAL ISOLATES FROM RAW MILK AND DAIRY PRODUCTS IN RIYADH REGION AND THEIR SUSCEPTIBILITY TO COMMON ANTIBIOTICS

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Last updated: 04 Jan 2025

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Abstract

A total of 100 samples of raw milk, various cheeses, labnah, yogurt, and egett were collected from appropriate suppliers and markets in Riyadh region. Bacteriological analysis for typing of enterococci and other lactic Acid Bacteria (LAB) was carried out by plating appropriate dilutions of each sample on sheep blood agar and Edwards blood agar plates. After overnight aerobic incubation at 37°, the presumptive identification was done by colony morphology, cultural characteristics, Gram-stain and catalase production. Final identification to the genera and species level of the total 125 bacterial isolates was completed by API-20 strips as well as Lancefield-serogrouping. Results revealed that Enterococcus faecium (88 isolates) accounts of 70% of total bacterial isolates, while Enterococcus faecalis (26 isolates) accounts of up to 21% and other LAB constituted about 9% of total recovered isolates. The later isolates comprises 3, 3, and 5 isolates of Enterococcus gallinarum, Enterococcus durans, and Aerococcus viridans respectively. The results revealed that nature of sample, its pH, and salinity clearly affect the incidence and number of recovered bacterial isolates. Thus as pH rises towards neutrality, with no salt or low salinity, E. faecalis and other LAB were recovered more frequently, and vice versa. In contrast, E. faecium was routinely isolated from most of the examined samples regardless of their pH range and salinity- content, reflecting its ubiquitous nature and its tolerance to drastic environmental conditions, thereby facilitating person to person transmission. The dominance or persistence of enterococci in examined samples is most probably attributed to their wide range of growth temperatures, their tolerance to heat, salt and acid. In addition, the MIC of each of the tested 120 isolates was determined by serial dilution in Muller Hinton sheep blood agar against 9 antibiotics. All isolates were sensitive to ampicilin with the exception of one E. faecalis strain that showed an MIC of 4 ug/ml. While Erythromycin (EM) exhibited also a good activity with an MIC50/MIC90 of 1/1, 1/4, 2/8 and 4/4 ug/ml. for E. gallinarum, E. faecalis, E. faecium, and E. durans or Aerococcus viridans isolates respectively. Whereas all isolates were resistant to cefoxitin and about 50% were also resistant to Chloramphenicol (CM), Tetracycline (TC), or Trimethoprim / Sulfamethoxazole (SXT). Thus at the breakpoint of MIC (>16 ug/ml.) (TC) resistance rate for E. faecalis was 16% and for E. faecium and E. gallinarum was 35% and 100% respectively. Whereas that for vancomycin (VM) the figures were 44%, 19% and 100% respectively. It is concluded that the examined samples may constitute a potential source for the dissemination of antibiotic resistant determinants to human.

DOI

10.21608/bfsa.2004.65430

Authors

First Name

L.

Last Name

Nasser

MiddleName

A.

Affiliation

Girl's College of Education, Riyadh, Saudi Arabia

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Orcid

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First Name

T.

Last Name

Elkersh

MiddleName

A.

Affiliation

Department of Clinical laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia

Email

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City

-

Orcid

-

First Name

S.

Last Name

Mejally

MiddleName

H.

Affiliation

Department of Clinical laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia

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Volume

27

Article Issue

1

Related Issue

9857

Issue Date

2004-06-01

Receive Date

2004-03-13

Publish Date

2004-06-30

Page Start

133

Page End

140

Print ISSN

1110-0052

Online ISSN

3009-7703

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https://bpsa.journals.ekb.eg/article_65430.html

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https://bpsa.journals.ekb.eg/service?article_code=65430

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17

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

ENTEROCOCCAL ISOLATES FROM RAW MILK AND DAIRY PRODUCTS IN RIYADH REGION AND THEIR SUSCEPTIBILITY TO COMMON ANTIBIOTICS

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Article

Created At

22 Jan 2023