Biological evaluation of natural preparations used for treatment of hair loss revealed that the saponifiable fraction was the active fraction. The main constituents of this fraction were palmitic, stearic saturated fatty acids and oleic and linoleic unsaturated fatty acids. Certain modifications were done to increase the activity by addition of Cantharidin or Pilocarpine to the most active subfraction of saponifiable matter. The superior effect on treating hair loss was shown with the formulation containing cantharidin than that which contain pilocarpine