Three phenylethanoid glycosides viz; -[(3′,4′-dihydroxyphenyl)-ethyl]-(4′َ′-O-caffeoyl)--
D-glucoside (desrhamnosylacteoside) (1),  -[(3′,4′-dihydroxyphenyl)-ethyl]-(3′′-O-Lrhamnosyl)-(
4′′-O-caffeoyl)- -D-glucoside (acteoside) (2) and  -[(3′,4′-dihydroxyphenyl)-
ethyl]-(3′′,6′′-O-L-dirhamnosyl)-(4′′-O-caffeoyl)- -D-glucoside (poliumoside) (3) were isolated
and identified from the callus cultures of Barleria cristata L.The structures of the isolated
compounds were established by spectroscopic evidence (UV, 1D and 2D-NMR and ESIMS),
further confirmation has been done by comparison with authentic samples. The amount of
compounds 1-3 were determined in the callus culture using HPLC.