From the chloroform soluble fraction of the methanolic extract of the corms of Gladiolus segetum Ker-Gawl (Iridaceae), nine compounds were isolated and identified as follows: the lignans (+)-demethoxypinoresinol (1), (+)-pinoresinol (2) and (+)-pinoresinol monomethylether (3); the neolignan (–)-dehydrodiconiferyl alcohol (4) and the anthraquinones deoxyerythrolaccin (5), physcion (6) and laccaic acid D methylester (7) together with 6′-O-palmitoyl3-O -sitosterol glucoside (8) and  -sitosterol-3-O-glucoside (9). The structures of the isolated compounds were determined by physical and spectroscopic methods including NMR and MS spectral analysis. Compounds 1-4 and 6-9 are reported here for the first time from the genus Gladiolus while compound 5 was previously isolated from the same plant.