Novel Schiff bases of 4-methyl-1,2,4-triazole-3-mercaptoacetic acid hydrazide were synthesized. Their chemical identities were elucidated by elemental analyses, IR, 1H-NMR, 13C-NMR and mass spectral data. The percentage of the geometrical isomers was also elucidated using the 1H-NMR. The synthesized compounds were selected for screening at the Tuberculosis Antimicrobial Acquisition and Coordination Facility (TAACF) against Mycobacterium tuberculosis H37Rv strain in which they showed moderate activity at a concentration of 6.25  g/ml. Moreover, antimicrobial screenings against E. coli (ATCC 25922), S. aureus (ATCC 19433) and C. albicans identified compound 4g as the most effective showing similar antibacterial activity as ampicillin against S. aureus.