In order to know the prevalence of Aeromonas species as a causative agent of diarrhea in Ismailia, Three hundreds and fifty stool samples and rectal swabs were collected from infants and children under the age of 5 years visiting El Kilo11Clinic suffering from acute diarrhea in the period from May 2004 to September 2004. A stool samples were collected from control group of fifty healthy infants and children of matched age and sex. It was found that 29 diarrheal samples were positive for Aeromonas species with prevalence of 8.28%. None of control samples were positive for Aeromonas. In most cases it was found that the infection is water or food borne. Aeromonas strains show high degree of antibiotic susceptibility.