Fractionation and purification of the alcoholic extract of the leaves and stem -bark of Vangueria edulis cultivated in Egypt afforded thirteen compounds identified as: β-sitosterol acetate (1), stigmasterol (2), palmitic acid (3), scopoletin (4), p-coumaric acid (5), protocatechuic acid (6), esculetin (7), ethyl 1-O-glucosyl-4-O-(E) caffeoyl quinate (8), kaempferol 3-O-rhamnoside, 7-O-rutinoside (9), β-sitosterol (10), ceryl alcohol (11), vanillic acid (12) and β-sitosterol-3-O-β-D-glucopyranoside (13). Identification of these compounds has been established by physical, chemical and spectral data as well as comparison with authentic samples. Compounds 8 and 9 are firstly reported from a natural source while the rest of the compounds have been isolated for the first time from the genus Vangueria. The LD50, antibacterial, antifungal, anti-inflammatory, antipyretic and the effect on CNS were studied.