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MICROBIOLOGICAL ASSAY OF COLISTIN SULFATE ANTIBIOTIC IN PHARMACEUTICAL FORMULATIONS

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Last updated: 04 Jan 2025

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Abstract

A simple, sensitive and specific agar cup diffusion bioassay for the antibacterial Colistin
sulfate was developed. Using a strain of Escherichia coli ATCC 8739 as the test organism,
Colistin sulfate at concentrations ranging from 100 to 1600 μg/ml could be measured in
pharmaceuticals. A prospective validation of the method showed that the method was linear
(r2= 0.999), precise (RSD< 2.8%) and accurate (percent recovery ranges between 98-102%).
The method shows that results confirm its precision, not differing significantly from the other
method described in the literature. We conclude that microbiological assay is satisfactory using
Escherichia coli ATCC 8739 for quantitation of in-vitro antibacterial activity of Colistin sulfate.

DOI

10.21608/bfsa.2013.63200

Authors

First Name

Omnia

Last Name

Albadawy

MiddleName

H.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71526, Egypt

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Orcid

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First Name

Amany

Last Name

Nafee

MiddleName

M.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71526, Egypt

Email

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City

-

Orcid

-

First Name

Momen

Last Name

Thabet

MiddleName

-

Affiliation

CID Company, Assiut, Egypt

Email

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City

-

Orcid

-

First Name

Mostafa

Last Name

El-Rehewy

MiddleName

S.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71526, Egypt

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Ahmed

MiddleName

S.

Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut 71526, Egypt

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Volume

36

Article Issue

2

Related Issue

9554

Issue Date

2013-12-01

Receive Date

2013-06-23

Publish Date

2013-12-01

Page Start

93

Page End

103

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 36_Issue 2_Pages 93-103.pdf

PDF . 1.5MB

Link

https://bpsa.journals.ekb.eg/article_63200.html

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https://bpsa.journals.ekb.eg/service?article_code=63200

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

MICROBIOLOGICAL ASSAY OF COLISTIN SULFATE ANTIBIOTIC IN PHARMACEUTICAL FORMULATIONS

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Article

Created At

22 Jan 2023