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63196

ESTABLISHMENT OF CALLUS AND CELL SUSPENSION CULTURES OF CASSIA BICAPSULARIS L.

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Last updated: 25 Dec 2024

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Abstract

Callus cultures were initiated from leaf of Cassia bicapsularis L. on solid Murashige &
Skoog (MS) basal medium supplemented with different growth regulators. Excellent growth of
callus was obtained in medium supplemented with 1 mg/l 2,4-dichlorophenoxyacetic acid (2,4-
D) and grown in the dark. The obtained callus was subcultured every 4 weeks in the dark at
25°C. The Callus was compact, yellowish brown in colour and used for establishment of cell
suspension cultures. Maximum growth of suspension cultures was achieved in medium
supplemented with 1 mg/l 2,4-D and 0.1 mg/l kinetin. The growth rate of cells was initially slow
but as the cultures proceeded, the growth increased significantly over a period of 22 days then
the growth of cells was stable for 35 days.

DOI

10.21608/bfsa.2013.63196

Authors

First Name

Iman

Last Name

Abdel-Rahman

MiddleName

A. M.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt , Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Mendelssohnstrasse 1, 38106 Braunschweig, Germany

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Orcid

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First Name

Afaf

Last Name

Abdel-Baky

MiddleName

M.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

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-

City

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Orcid

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First Name

Ezz El-Din

Last Name

Desoky

MiddleName

K.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

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-

City

-

Orcid

-

First Name

Amany

Last Name

Ahmed

MiddleName

S.

Affiliation

Department of Pharmacognosy, Faculty of Pharmacy, Assiut University, Assiut 71526, Egypt

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City

-

Orcid

-

First Name

Ludger

Last Name

Beerhues

MiddleName

-

Affiliation

Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Mendelssohnstrasse 1, 38106 Braunschweig, Germany

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Orcid

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Volume

36

Article Issue

1

Related Issue

9553

Issue Date

2013-06-01

Receive Date

2013-01-09

Publish Date

2013-06-01

Page Start

23

Page End

30

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 36_Issue 1_Pages 23-30.pdf

PDF . 163.5kB

Link

https://bpsa.journals.ekb.eg/article_63196.html

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https://bpsa.journals.ekb.eg/service?article_code=63196

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

ESTABLISHMENT OF CALLUS AND CELL SUSPENSION CULTURES OF CASSIA BICAPSULARIS L.

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Article

Created At

22 Jan 2023