The aim of this study was to evaluate compatibility and stability of the maximum
concentration used for binary admixture containing dobutamine and dopamine in 5% glucose.
The maximum concentration of each drug was 5.76 mg/ml of dobutamine and 2.88 mg/ml of
dopamine in 50 ml of 5% glucose. The physical compatibility of binary admixtures was assessed
using visual inspection and pH determination immediately after preparation (at 0 time) and
after 24 hrs. The chemical stability was assessed using high performance thin layer
chromatoghraphy (HPTLC). The method is based on HPTLC separation of the two drugs
followed by densitometric measurements of their spots at 254 nm using Camag TLC Scanner 3.
The mobile phase comprised ethyl acetate : n-propanol : water : glecial acetic acid (60:24:9:3,
v/v/v/v). The results revealed that no precipitation, gas evaluation, color change, pH change or
chemical incompatibility were observed over the entire time of mixing of two drugs in 5%
glucose solution.