ManageChatDiscover
235237

Hemagglutinin Gene Diversity of Canine Distemper Virus from Clinical Samples in Brazil

Article

Last updated: 04 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Virology

Abstract

Canine distemper virus (CDV) affecting both domestic dogs and wild animals, resulting in high rates of morbimortality. The viral genome encodes six different proteins where the Hemagglutinin is the most variable, being the basis for molecular phylogenetic analysis and may lead to antibody recognition mismatch and vaccine failure. The study aimed to analyze the H gene of the CDV in field samples from naturally infected dogs in Brazil. A total of 24 blood, urine and ocular discharge samples were collected from naturally infected dogs from endemic areas of Brazil. RNA from clinical samples of infected animals was extracted, PCR products of H gene amplification were aligned, the sequences of samples were submitted to a public database search and were aligned with the protein sequence of the Onderstepoort CDV vaccine strain to explore their similarity profiles. Sequencing of the fragments that represent the entire H gene sequence demonstrated five distinct isolates. The phylogenetic analysis of the isolates showed that they belong to the cluster Europe/South America-1, differing substantially from the vaccine strains used in the country. Generated amino acids analyzed, showed that the specific substitutions at the SLAM receptor site of the H gene, previously linked to the emergence of diseases in new hosts, were not detected in these sequences. However, the R580Q substitution detected in the isolated strain from Pernambuco is considered imminently deleterious to the efficiency of fusion and expression of the surface of the binding protein. These findings demonstrate that the genetic variability identified in field strains of the distemper virus in endemic areas in Brazil shows genetic alterations that may lead to antibody mismatch.

DOI

10.21608/javs.2022.126513.1137

Keywords

Canine Morbillivirus, Hemagglutinin, Minas Gerais, Pernambuco, Phylogenetic analyzes

Authors

First Name

Vanessa Alessandra de

Last Name

Portela

MiddleName

Barros

Affiliation

Department of Veterinary Medicine, Federal Rural University of Pernambuco, Dom Manoel de Medeiros Street, s/n, Recife-PE, Brazil

Email

alessandra.barros.portela@gmail.com

City

-

Orcid

0000-0001-7670-9153

First Name

Klarissa

Last Name

Guarines

MiddleName

Miranda

Affiliation

Aggeu Magalhães Institute - IAM/FIOCRUZ-PE, Av. Prof. Moraes Rego, s/n - Cidade Universitária, Recife - PE, Brazil

Email

klarissaguarines@gmail.com

City

-

Orcid

0000-0003-4225-2890

First Name

Cláudia Kathariny da

Last Name

Farias

MiddleName

Silva

Affiliation

Department of Veterinary Medicine, Federal Rural University of Pernambuco, Dom Manoel de Medeiros Street, s/n, Recife-PE, Brazil

Email

claudiakfarias@hotmail.com

City

-

Orcid

0000-0001-6828-8731

First Name

Otávio

Last Name

Carvalho

MiddleName

Valério de

Affiliation

Tecsa Laboratories, Av. do Contorno, 6226 - Funcionários, Belo Horizonte - MG, Brazil

Email

otaviovet@gmail.com

City

-

Orcid

0000-0003-1282-0084

First Name

Edmilson

Last Name

Oliveira-Filho

MiddleName

Ferreira de

Affiliation

Charité - Universitätsmedizin Berlin, corporate member of FreieUniversität Berlin, Humboldt-Universitätzu Berlin, and Berlin Institute of Health, Institute of Virology, Charitépl. Berlin, Germany

Email

edmilsonz@gmail.com

City

-

Orcid

0000-0002-3771-1565

First Name

Adalucia da

Last Name

Silva

MiddleName

-

Affiliation

Aggeu Magalhães Institute - IAM/FIOCRUZ-PE, Av. Prof. Moraes Rego, s/n - Cidade Universitária, Recife - PE, Brazil

Email

adaluciasilva9@gmail.com

City

-

Orcid

0000-0001-8047-8538

First Name

Lindomar

Last Name

Pena

MiddleName

José

Affiliation

Aggeu Magalhães Institute - IAM/FIOCRUZ-PE, Av. Prof. Moraes Rego, s/n - Cidade Universitária, Recife - PE, Brazil

Email

lindomarvet10@gmail.com

City

-

Orcid

0000-0003-1134-5699

First Name

Rita de Cassia

Last Name

Maia

MiddleName

Carvalho

Affiliation

Department of Veterinary Medicine, Federal Rural University of Pernambuco, Dom Manoel de Medeiros Street, s/n, Recife-PE, Brazil

Email

r.carvalhomaia@gmail.com

City

-

Orcid

0000-0001-6765-6686

Volume

7

Article Issue

3

Related Issue

35409

Issue Date

2022-07-01

Receive Date

2022-03-11

Publish Date

2022-07-09

Page Start

1

Page End

11

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 7_Issue 3_Pages 1-11.pdf

PDF . 1.0MB

Link

https://javs.journals.ekb.eg/article_235237.html

Detail API

https://javs.journals.ekb.eg/service?article_code=235237

Order

1

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Hemagglutinin Gene Diversity of Canine Distemper Virus from Clinical Samples in Brazil

Details

Type

Article

Created At

22 Jan 2023