In this study, the duration of immunity as well as maternally derived antibodies (MDA) induced by the innovated tissue culture pigeon pox vaccine (TC-PPV) were evaluated for the first time. It was found that the postvaccinal reaction was more prominent in pigeons vaccinated with egg-adapted PPV (EG-PPV) (100%) than in pigeons vaccinated with TC-PPV (88%). The duration of induced immunity was higher using EG-PPV (1 year with a peak of neutralizing antibodies index 3.25 while it was 2.75 using TC-PPV) after 1 month of vaccination. MDA in squabs of vaccinated dams with TC-PPV maintained the protective level of antibodies up to 3weeks post-hatching (WPH) with 100% protection against a challenge. In contrast, in squabs of vaccinates with EG-PPV, it remained up to 4 WPH with 90 % protection. Such antibody levels hinder the immune response to vaccination with TC-PPV resulting in vaccination failure with 40% protection for squabs of TC-PPV vaccinated dams at 3^rd WPH and 60% protection for squabs of EG-PPV vaccinated dams at 4^th WPH reflecting the successive vaccination at the 4^th WPH and 5^th WPH, respectively for hatched squabs with 80% protection in both groups while in squabs of unvaccinated dams; the vaccination with TC-PPV appears to be protective from ^2nd WPH with 100% protection. In addition, this study recommends revaccination of pigeons with TC-PPV after 10 months and vaccination of their squabs not before 4 WPH to avoid vaccination failure by MDA.