Bacteria are seriously affecting broiler chickens and the poultry industry in many countries, as well as in Egypt. Both pathogenic and nonpathogenic bacterial species have been reported in broiler chickens. The present study aimed to isolate and identify the most common bacteria recovered from apparently healthy broiler chickens by conventional methods and the VITEK system. Samples were taken from 30 broiler chickens of different ages (3 – 5 weeks), from different retail markets in Cairo, during the period from January to August 2019. Bacteriological examination of the samples showed both Gram positive and Gram negative bacteria as well. Lysinibacillus sphaericus (n=7), Escherichia coli (n = 5), Proteus species (n = 4), Bacillus cereus (n = 3), with isolation rates of 23.3 %, 16.6 %, 13.3 % and 10 %, were identified. In addition to its pathogenicity and hazardous action, some strains of Lysinibacillus sphaericus have a larvicide effect on some species of mosquitoes. So, further studies and investigations will be conducted to test Lysinibacillus sphaericus and Bacillus cereus isolates for their biological control activity and / or their potency to remove, control, or reduce petroleum hydrocarbon soil contaminants in different localities. It will be used as an alternative control agent for numerous synthetic commercial formulations.