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190080

Characterization of Egyptian Isolates of Canine Distemper and Canine Parvoviruses

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Virology

Abstract

This study was aimed to investigate the incidence of CD and CP viruses in Cairo, Egypt. In a private Vet. Clinic in Cairo, five local breed puppies of about 3-5 months of age were supposed to be infected with the virus of canine distemper (CDV) showing fever, runny nose, salivation and coughing. Another 6 German Shepard and 4 Gryphon puppies of about 6-9 months of age were suffered from fever and bloody diarrhea suspected to be infected with canine parvovirus (CPV). All of these puppies had no history of vaccination. Trials of virus segregation in Vero and MDCK cell lines and usage of virus neutralization test (VNT) using specific anti-CD and anti-CP sera revealed that 3 out of 5 nasal swabs were positive to CD virus and 3 out of 10 fecal swabs were positive to CPV. These results were confirmed by Rt-PCR showing positive amplification with CD and CP, respectively. So, we could say that VNT and Rt-PCR are essential assays to identify CD and CP viruses and puppy vaccination should not be neglected to protect them against such diseases.

DOI

10.21608/javs.2021.84062.1088

Keywords

canine distemper, canine parvo, Fluorescent antibody technique, RT-PCR

Authors

First Name

M.

Last Name

Khodeir

MiddleName

H.

Affiliation

Veterinary Serum and Vaccine Research Institute, Agriculture Research Center, Abasia,Cairo, Egypt.

Email

profdrkhodeir@gmail.com

City

cairo

Orcid

-

First Name

Elbehwar

Last Name

A.M.

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Agriculture Research Center, Abasia,Cairo, Egypt.

Email

behwara@gmail.com

City

cairo

Orcid

-

First Name

Omaima

Last Name

El-Shamandy

MiddleName

A.E.

Affiliation

Veterinary Serum and Vaccine Research Institute, Agriculture Research Center, Abasia,Cairo, Egypt.

Email

ashraf.eldakhly2000@gmail.com

City

Cairo

Orcid

-

First Name

Soliman

Last Name

Y.A.

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center, Abasia, Egypt.

Email

soliman.eldakhly@yahoo.com

City

cairo

Orcid

-

First Name

El-dakhly

Last Name

A.T.

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute, Agriculture Research Center, Abasia,Cairo, Egypt.

Email

ashraf.eldakhly@yahoo.com

City

cairo

Orcid

0000-0003-1324-7988

First Name

Sahar

Last Name

M. Saber

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Agriculture Research Center, Abasia, Egypt.

Email

sahar.saber@yahoo.com

City

cairo

Orcid

-

Volume

6

Article Issue

4

Related Issue

27718

Issue Date

2021-10-01

Receive Date

2021-07-05

Publish Date

2021-10-01

Page Start

1

Page End

6

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 6_Issue 4_Pages 1-6.pdf

PDF . 658.6kB

Link

https://javs.journals.ekb.eg/article_190080.html

Detail API

https://javs.journals.ekb.eg/service?article_code=190080

Order

1

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Characterization of Egyptian Isolates of Canine Distemper and Canine Parvoviruses

Details

Type

Article

Created At

22 Jan 2023