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154576

Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Bacteriology

Abstract

Corynebacterium pseudotuberculosis is a small Gram-positive bacillus containing mycolic acid in the structure of the cell wall. The bacterium is responsible for Caseous Lymphadenitis (CLA) in small ruminants (sheep and goats). The bacteria are also responsible for Ulcerative Lymphangitis in equines. The disease causes great economic loss in the animal industry. This work aimed to check the ability of Quadruplex PCR (Q-PCR) for genotyping and identification of Egyptian isolates of C. pseudotuberculosis and sequence analysis of phospholipase D (PLD) gene of local isolates. Four of C. pseudotuberculosis local isolates previously biochemically identified were tested for narG gene (nitrate reductase gene). Both nitrate negative biovar (ovis) and nitrate positive biovar (equi) showed a positive result for 16S rRNA, rpoB and PLD genes of C. pseudotuberculosis species. The sequence analysis of our local isolates' PLD gene revealed minor changes in PLD proteins between ovine and equine strains compared with other published PLD sequences in GenBank. It was concluded that the Q-PCR method is able to differentiate between C. pseudotuberculosis equi and ovis biovars. Also, the sequence of PLD gene of local isolates representing the two biovars revealed some variation, which leads to an accurate diagnosis of C. pseudotuberculosis biovars and generates a mapping of immerged local isolates. Further, the PCR and sequence of these isolates provide rapid and accurate genotyping, especially with hyperimmune serum's unavailability.

DOI

10.21608/javs.2021.154576

Keywords

C. pseudotuberculosis, PLD gene, Quadruplex PCR, sequence analysis

Authors

First Name

Nadine

Last Name

El-Sebay

MiddleName

A.

Affiliation

Genetic Engineering Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Egypt.

Email

nsebaie@gmail.com

City

-

Orcid

-

First Name

Marwah

Last Name

Abd Elmonem

MiddleName

M.

Affiliation

Bacterial diagnostic products department, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Egypt.

Email

ryym_110@yahoo.com

City

Cairo

Orcid

-

First Name

Elham

Last Name

El-Sergany

MiddleName

F.

Affiliation

Anaerobic Bacterial Vaccine Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Egypt.

Email

elham1832@hotmail.com

City

-

Orcid

-

First Name

Ashraf

Last Name

M. Abbas

MiddleName

-

Affiliation

Genetic Engineering Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Egypt.

Email

-

City

-

Orcid

-

First Name

Roukaya

Last Name

M. Osman

MiddleName

-

Affiliation

Anaerobic Bacterial Vaccine Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Egypt.

Email

-

City

-

Orcid

-

First Name

Dalia

Last Name

Abd El-Moaty

MiddleName

A. M.

Affiliation

Genetic Engineering Research Department, Veterinary Serum and Vaccine Research Institute (VSVRI), Agriculture Research Center (ARC), Egypt.

Email

dody.ahmed@gmail.com

City

-

Orcid

-

Volume

6

Article Issue

2

Related Issue

22649

Issue Date

2021-04-01

Receive Date

2021-01-07

Publish Date

2021-04-01

Page Start

15

Page End

21

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 6_Issue 2_Pages 15-21.pdf

PDF . 486.8kB

Link

https://javs.journals.ekb.eg/article_154576.html

Detail API

https://javs.journals.ekb.eg/service?article_code=154576

Order

2

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Genetic Characterization of Corynebacterium pseudotuberculosis Isolates in Egypt

Details

Type

Article

Created At

22 Jan 2023