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140096

Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid

Article

Last updated: 25 Dec 2024

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-

Tags

Virology

Abstract

A combined vaccine of Equine influenza (EI), equine herpesvirus–1 (EHV-1), rabies virus and tetanus toxoid adjuvanted with saponin and Alhydrogel was prepared. Quality control testing of such vaccine revealed that it was free from foreign contaminants, safe for Guinea-pigs, mice and mares. It was inoculated into Guinea -pigs as a preliminary evaluation for its potency and it was proven safe and potent. Also, it exceeded the permissible protective level allowed to be used for the vaccination of horses. Two groups of mares were used for evaluating the potency of the vaccine. The first one received two doses (5 ml) of the prepared vaccine with 4 weeks interval while the 2nd group was kept without vaccination as control. The mean haemagglutination inhibition (HI) influenza antibody titer reached its maximum at the 2nd month post-vaccination (MPV); 2048 while EHV-1 antibodies reached the peak at the 3rd MPV as recorded by ELISA and neutralizing indices; 1790 and 3.50. Rabies antibodies were detectable in vaccinated mares by the 2nd week post-vaccination showing titers of 16 and 1.0 by serum neutralization test (SNT) and ELISA, respectively recording their peaks (128 by SNT and 2.15 by ELISA) at the 2nd MPV. Tetanus antitoxic titer increased till reaching the peak at the 2nd MPV (40IU/ml) as determined by toxin neutralization test. Depending on these results, it could be concluded that the prepared inactivated EI, EHV-1, rabies and tetanus vaccine is safe and potent for mares providing them with good protective levels of specific antibodies against the 4 used antigens up to not less than 6 months.

DOI

10.21608/javs.2021.140096

Keywords

vaccine, Equine influenza, herpesvirus-1, rabies, Tetanus

Authors

First Name

Safaa

Last Name

A. Warda

MiddleName

-

Affiliation

Department of Equine Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbassia, Cairo, Agriculture Research Center (ARC), Egypt.

Email

-

City

-

Orcid

-

First Name

wafaa

Last Name

abdelaziz

MiddleName

R.

Affiliation

Department of Sera and antigens Research , Veterinary Serum and Vaccine Research Institute (VSVRI), Abbassia, Cairo, Agriculture Research Center (ARC), Egypt.

Email

dr.wafaa3@gmail.com

City

-

Orcid

0000-0003-1597-904X

First Name

Eman

Last Name

M. Ebied

MiddleName

-

Affiliation

Department of Equine Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbassia, Cairo, Agriculture Research Center (ARC), Egypt.

Email

-

City

-

Orcid

-

First Name

Nashwa

Last Name

K. Madkour

MiddleName

-

Affiliation

Department of Equine Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbassia, Cairo, Agriculture Research Center (ARC), Egypt.

Email

-

City

-

Orcid

-

First Name

Hala

Last Name

El Sawy

MiddleName

-

Affiliation

Department of In-House Quality Control , Veterinary Serum and Vaccine Research Institute (VSVRI), Abbassia, Cairo, Agriculture Research Center (ARC), Egypt.

Email

-

City

-

Orcid

-

First Name

Fatma

Last Name

F.Warda

MiddleName

-

Affiliation

Department of Equine Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbassia, Cairo, Agriculture Research Center (ARC), Egypt.

Email

-

City

-

Orcid

-

First Name

Omaima

Last Name

A.E. El-Shamandy

MiddleName

-

Affiliation

Department of Pet Animal Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbassia, Cairo, Agriculture Research Center (ARC), Egypt.

Email

-

City

-

Orcid

-

First Name

Attyat

Last Name

M. Kotb

MiddleName

-

Affiliation

Department of Pet Animal Vaccine Research, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbassia, Cairo, Agriculture Research Center (ARC), Egypt.

Email

-

City

-

Orcid

-

Volume

6

Article Issue

1

Related Issue

20722

Issue Date

2021-01-01

Receive Date

2020-12-07

Publish Date

2021-01-16

Page Start

38

Page End

44

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 6_Issue 1_Pages 38-44.pdf

PDF . 610.2kB

Link

https://javs.journals.ekb.eg/article_140096.html

Detail API

https://javs.journals.ekb.eg/service?article_code=140096

Order

6

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Trials For Preparation Of Combined Vaccine Of Equine Influenza, Equine Herpesvirus–1, Rabies Virus And Tetanus Toxoid

Details

Type

Article

Created At

22 Jan 2023