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101927

Preparation and Evaluation 0f A Recent Infectious Laryngotracheitis (LIT) Vaccine From A Local Field Isolate

Article

Last updated: 25 Dec 2024

Subjects

-

Tags

Poultry diseases

Abstract

Infectious laryngotracheitis (ILT) is a respiratory tract disease affecting chickens worldwide. The disease leads to severe production losses due to increased mortality, decreased egg production, and delayed body weight gain, causing enormous economic losses to the poultry industry. ILT is controlled through vaccination with live-modified attenuated vaccines of chicken embryo origin (CEO) and/or the tissue-culture origin (TCO). This study was conducted to develop Specific Pathogenic Free (SPF) egg-adapted live attenuated ILT vaccine from the field strain Fayoum_2018 isolated from broilers in Fayoum Province, Egypt in 2018. The isolate Fayoum_2018 with accession number (MN082684) was characterized molecularly as a TCO vaccine related strain. Measurement of immunity in vaccinated chicks employing the potency test revealed that chicks vaccinated with 0.5 ml of prepared ILT vaccine exhibited ELISA with 1905 antibody titer at 2nd-week post-vaccination, which was considered an ILT adequate immune response and increased till reach 3497 antibody titer at 6th-week post-vaccination. The current work revealed that Fayoum_2018 ILTV egg adapted live attenuated vaccine produced a satisfactory antibodies titer that was efficient in controlling the ILT field infection in Egypt.

DOI

10.21608/javs.2020.101927

Keywords

BVDV, ILT, CEO, TCO, SPF, ELISA

Authors

First Name

Nada

Last Name

Fathy

MiddleName

A.

Affiliation

Department of Newcastle disease vaccine research, Veterinary Serum and Vaccines Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo, Egypt.

Email

nadadl@yahoo.com

City

-

Orcid

-

First Name

Abd El-Moneam

Last Name

M.M.

MiddleName

-

Affiliation

Department of Newcastle disease vaccine research, Veterinary Serum and Vaccines Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo, Egypt.

Email

dr_mahmoud_vsvri@yahoo.com

City

-

Orcid

-

First Name

Ibrahim

Last Name

S.

MiddleName

-

Affiliation

Department of Genetic Engineering, Veterinary Serum and Vaccines Research Institute (VSVRI), Agricultural Research Center (ARC), Abbasia, Cairo, Egypt

Email

sherifmmm@hotmail.com

City

-

Orcid

-

First Name

Nourhan

Last Name

Mohamed

MiddleName

Nagy

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics (CLEVB)El-Seka El-Baida, Abbasia, Cairo, Egypt

Email

nouranagy74@yahoo.com

City

-

Orcid

-

First Name

Amina

Last Name

Radwan

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Abbasia, Cairo , Egypt.

Email

aminar9066@gmail.com

City

-

Orcid

-

First Name

Rafik

Last Name

Sayed

MiddleName

Hamed

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Agricultural Research Center (ARC), Abbasia, Cairo , Egypt

Email

rafikhamed1010@hotmail.com

City

-

Orcid

-

Volume

5

Article Issue

3

Related Issue

12904

Issue Date

2020-07-01

Receive Date

2020-06-09

Publish Date

2020-07-01

Page Start

87

Page End

91

Print ISSN

1687-4072

Online ISSN

2090-3308

Link

https://javs.journals.ekb.eg/article_101927.html

Detail API

https://javs.journals.ekb.eg/service?article_code=101927

Order

10

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Preparation and Evaluation 0f A Recent Infectious Laryngotracheitis (LIT) Vaccine From A Local Field Isolate

Details

Type

Article

Created At

22 Jan 2023