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85638

The Immunogenicity and Protective Efficacy of DNA Vaccine Coding For NA1 Gene of Highly Pathogenic Avian Influenza H5N1 Subtype

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Immunology and Vaccinology

Abstract

Control of avian influenza infection depends mainly on biosafety measures and vaccination, DNA vaccination is a novel method to generate antigen-specific antibody and cell-mediated immunity. In the current study, a DNA vaccine for a full-length N1 gene was developed in a trial to decrease the severity of the avian influenza virus spread and shedding. THE full-length N1 gene was cloned in entry clone p < /em>ENTER SD/TOPO, followed by homologous recombination with the destination mammalian expression vector (PDEST 40). The PDEST 40-N1 was used in the immunization of SPF chickens. Potency was evaluated through the survival rate that reaches 65%, which was far less than the commercially available inactivated vaccine. Meanwhile, the shedding of the virus from dead birds was 0.46 Log 10 EID50. At the same time, the most surprising result was the shedding level of the vaccinated live birds that were zero sheddings;on the other hand, the inactivated vaccine could not reduce the shedding level which remains very high (3.2 Log 10 EID50 ). IFN-γ transcript level in the DNA vaccinated group was detected by the 3rd-day post-vaccination and remained upregulated till the 28th post-vaccination. After the challenge, the level of IFN-γ was much higher until 14 days post-challenge. The inactivated vaccine could not stimulate any detectable level post-vaccination. These data suggested the ability of DNA vaccine coding for N1 gene of avian influenza to combat the virus shedding from live birds and could be used in combination with DNA vaccine coding for H5 to produce maximum protection with zero sheddings.

DOI

10.21608/javs.2020.85638

Authors

First Name

Soliman

Last Name

YA

MiddleName

-

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics ,Abbasiaa ,Cairo, Egypt.

Email

dryousefadel@gmail.com

City

-

Orcid

-

First Name

Maha

Last Name

Gamal

MiddleName

NA

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics, Abbasia ,Cairo, Egypt.

Email

mahaaboelnaga87@gmail.com

City

-

Orcid

-

First Name

Eman

Last Name

EL-Nagar

MiddleName

-

Affiliation

Veterinary Serum and Vaccine Research Institute VSVRI , Abbasia Cairo, Egypt.

Email

emanalnagar81@gmail.com

City

-

Orcid

-

First Name

Khalil

Last Name

S.

MiddleName

-

Affiliation

Faculty of Vet. Med., Alexandria University, Alexandria, Egypt.

Email

khaliel1@yahoo.com

City

-

Orcid

-

Volume

5

Article Issue

2

Related Issue

12838

Issue Date

2020-04-01

Receive Date

2020-04-06

Publish Date

2020-04-25

Page Start

109

Page End

123

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 5_Issue 2_Pages 109-123.pdf

PDF . 1.5MB

Link

https://javs.journals.ekb.eg/article_85638.html

Detail API

https://javs.journals.ekb.eg/service?article_code=85638

Order

14

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

The Immunogenicity and Protective Efficacy of DNA Vaccine Coding For NA1 Gene of Highly Pathogenic Avian Influenza H5N1 Subtype

Details

Type

Article

Created At

22 Jan 2023