The goal of the present study is to evaluate The Evaluation the effect of Analgesic Activity of Coenzyme Q10 ointment in mice. Materials and Methods: Fifteen healthy male albino mice were selected for this study. The animals were divided into three groups of five animals for each group. Group 1 served as a control and was applied vaseline ointment topically on fore and hind limb. Group 2 and 3 were applied topically Coenzyme Q10 ointment 4%, 8% respectively on fore and hind limb. The onset and duration of analgesic effect of Coenzyme Q10 ointment were evaluated in mice by utilizing a Hot-Plate test at 55±1˚C. Latency reaction time was recorded after 3min. and (10- 60 min) after ointment applied. The prolongation of latency times compared with the values of the control was used to express about analgesic effects of Coenzyme Q10 ointment and the percentage of Maximal Possible Effect (MPE) was calculated. Results: The Coenzyme Q10 ointment at concentrations (4%, 8%) produced analgesic effect in mice after 3 min, respectively in comparison with control. The percentage of maximum possible effect (MPE) was significantly increased in group treated with (4%,8%) Coenzyme Q10. Conclusions: The Coenzyme Q10 ointment have a good analgesic activity in mice after 3 minutes of topical application and prolong the duration of analgesia more than 40 minutes depending on concentration of Coenzyme Q10 ointment.