The present study aimed to prepare a combined inactivated  pneumo-4 vaccine containing bovine viral diarrhoea, infectious bovine rhinotracheitis (IBR), parainfluenza-3(PI-3) and bovine respiratory syncytial virus (BRSV)  adjuvanted with carbomere and compare the animal protection scope with its product mate Pneumo-4 adjuvanted alhydragel . Carbomere adjuvant stimulated higher levels and longer lasting antibody to pneumo-4 vaccine than vaccines of equivalent antigenic content containing aluminium hydroxide gel adjuvant. Quality control results proved that the pneumo-4 vaccine adjuvanted either by aluminium hydroxide gel adjuvant or carbomere (different concentration 0.2,0.5 and0.8 %)  was pure and completely safe to be used in calves without any abnomalities.Potency test was performed on five groups of five calves  for each group,Where the first group was vaccinated with pneumo-4 vaccine adjuvant with carbomere 0.2(formula 2), second group was vaccinated with pneumo-4 vaccine adjuvant with carbomere 0.5 (formula 3), third group was vaccinated with pneumo-4 vaccine adjuvant with carbomere 0.8(formula 4),,The fourth group vaccinated with pneumo-4 vaccine with aluminum hydroxide gel(formula 1),  and the fifth last  group was left as non vaccinated control group.compared humoral immune response  using serum neutralization and ELISA expressed by neutralization index of different vaccine formulation was revealed that The prepared pneumo-4 vaccine adjuvant with carbomere 0.5 proved to be highly potent formmula along 6 month after second booster dose applying. Also pneumo-4 vaccine adjuvant with carbomere  was showed  cellular immunity reaction higer than pneumo-4 vaccine with aluminum hydroxide gel using Estimation of nitric oxideactivity test.