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62675

Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Immunology and Vaccinology

Abstract

The present study was undertaken to development of BHK-21 cell adapted inactivated vaccine of Newcastle disease virus (NDVgenotype VII) from the field isolate from broiler chicken in Egypt during 2015-2016. The isolatesof El-Giza/2015were classified by sequencing as velogenic NDV genotype VII d contains F protein cleavage site motifs (112RRQKRF117). Such virus was propagated in the BHK-21 cell line. and cell adapted virus was confirmed as NDV by reverse transcription-polymerase chain reaction (RT-PCR) using fusion gene-specific primers and used to develop inactivated vaccine adjuvanted with Montanide IMS 1313.Potency test revealed that Vaccinated chicks with 0.5ml of prepared NDV vaccine exhibited HI antibody titer of 8.6 log2 three weeks post vaccination with the highest titer (10.6 log2/ml) at the 6th-week post vaccination, and 3rd weeks post challenge test. Protective antibodies values were persisting till 12th weeks post vaccination. All chicken groups vaccinated with both prepared inactivated tissue culture vaccine using ISA 1313 and VSVRI inactivated ISA70 adjuvant vaccines were passed challenge test (97.5%,97%,96% protective efficiency to SPF chickens) against the isolated virulent NDV, while the control group could not provide any protective efficiency. The present study indicated that, BHK-21 cell adapted recent isolated NDV inactivated vaccine produced a satisfactory antibodies titre that efficient in control of the disease in Egypt.

DOI

10.21608/javs.2019.62675

Keywords

Recent isolate, ISA, NDV, PCR

Authors

First Name

Amani

Last Name

Saleh

MiddleName

A.

Affiliation

Veterinary vaccine and serum research institute (VSVRI), El-Seka El-BaidaSt.Abbasia,Cairo,Egypt.

Email

-

City

-

Orcid

-

First Name

Nada

Last Name

Fathy

MiddleName

A.

Affiliation

Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo,

Email

nadadl@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

AbdRabo

MiddleName

A.

Affiliation

Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), El-Seka El-BaidaSt.Abbasia,Cairo,Egypt

Email

-

City

-

Orcid

-

First Name

Noha

Last Name

Helmy

MiddleName

A.

Affiliation

Veterinary vaccine and serum research institute (VSVRI), El-Seka El-BaidaSt.Abbasia,Cairo,Egypt.

Email

-

City

-

Orcid

-

Volume

4

Article Issue

1

Related Issue

9406

Issue Date

2019-04-01

Receive Date

2019-03-20

Publish Date

2019-04-12

Page Start

35

Page End

42

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 4_Issue 1_Pages 35-42.pdf

PDF . 532.7kB

Link

https://javs.journals.ekb.eg/article_62675.html

Detail API

https://javs.journals.ekb.eg/service?article_code=62675

Order

6

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Trials For Preparation And Evaluation Of Inactivated Tissue Culture Newcastle Disease Vaccine From Recent Isolate

Details

Type

Article

Created At

22 Jan 2023