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62123

Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth

Article

Last updated: 25 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

Virology

Abstract

This work presents cultural characterization of Peste Des Petites Ruminants (PPR) virus in VERO-culture. The VERO cells are currently considered as an acceptable cell substrate to produce a wide range of viruses. This study evaluates the best time for inoculation of PPR virus on VERO cell cultures; the study proved that the optimum time was 24 hours after subculture of VERO cell line using MOI 2: 1. It was also found that the best time of harvstation of virus fluid of PPR was 9th day post inoculation to reach the best titre 6 log10 TCID50 /ml.

DOI

10.21608/javs.2016.62123

Keywords

VERO cell, Peste Des Petits Ruminants, cell growth

Authors

First Name

A.

Last Name

El-Dakhly

MiddleName

T.

Affiliation

Rinder Pest Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt

Email

ashraf.eldakhly@yahoo.com

City

-

Orcid

0000-0003-1324-7988

First Name

M.

Last Name

Youssef

MiddleName

M.

Affiliation

Rinder Pest Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Abeer

Last Name

Tammam

MiddleName

A.

Affiliation

Rinder Pest Vaccine Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Namaa

Last Name

Moedham

MiddleName

A.

Affiliation

Pox Vaccines Research Department, Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

1

Article Issue

1

Related Issue

9357

Issue Date

2016-10-01

Receive Date

2016-07-10

Publish Date

2016-10-04

Page Start

69

Page End

72

Print ISSN

1687-4072

Online ISSN

2090-3308

Article File

JAVS_Volume 1_Issue 1_Pages 69-72.pdf

PDF . 428.4kB

Link

https://javs.journals.ekb.eg/article_62123.html

Detail API

https://javs.journals.ekb.eg/service?article_code=62123

Order

10

Type

Original Article

Type Code

1,095

Publication Type

Journal

Publication Title

Journal of Applied Veterinary Sciences

Publication Link

https://javs.journals.ekb.eg/

MainTitle

Assessment of the peste des petits ruminants (ppr) attenuated virus produced by inoculating vero cell line at different stages of cell growth

Details

Type

Article

Created At

22 Jan 2023