The present obtained experimental results proved that addition of lipoic acid (LA) with its sodium salt in ratio of 0.1ml mole (mM) in the growth media has improved their physiological activities of different cell lines including MDBK, VERO and BHK21. Such cells remained normal for relative longer time comparing with the cell culture provided with media without lipoic acid (LA). The improvement of used cell lines was ascribed to the addition of lipoic acid (LA) dihydrolipoic acid, (DHLA) as protein free media supplement. As Lipoic acid (LA) and its dihydrolipoic acid (DHLA) are involved in several aspects of cell energy and amino acid metabolism, as well as in defense against oxidative stress and apoptosis; both components initiated the production of glutathione which antagonizes the oxidative effect of free radicals on cells that harmfully affects cell metabolism .This study is in need to involve more investigation covering other uses of (LA) especially in its nano form.