Objectives: To evaluate the safety and efficacy of a commercially available absorbable barbed suture device in double-layer gastrotomy closure in a dog model. Methods: In this experimental comparative study a gastrostomy was performed in 12 adult healthy mongrel dogs (divided into two groups A and B). The stomach was closed using polyglactin 910 (Vicryle, Ethicon) suture in group A and using absorbable barbed suture (Stratafex, Ethicon) in group B. Results: Polyglactin 910 suture produced a more inflammatory reaction around the wound which caused a delay in gastric wound healing and gastric motility as well as gastric emptying time. The ultrasonographic examination revealed a significant increase in gastric wall thickness in group A compared to group B and a highly significant decrease of gastric contraction in group A in comparison to group B. Gastric closure time was significantly shorter in group B than in group A. No postoperative complications or deaths were observed in both groups. Conclusion: Absorbable barbed suture was recommended in gastric surgery as it is minimizing the time of gastric closure and optimizing the healing of the wound with faster returning of the stomach to its normal function.