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116236

EFFECT OF EXTENDERS ON THE CHARACTERISTIC AND FERTILITY OF COOLD BUFFALO SEMEN

Article

Last updated: 25 Dec 2024

Subjects

-

Tags

Veterinary Surgery and Theriogenology.

Abstract

The present study was conducted to investigate the effect of semen extenders onthe characteristics of cooled buffalo semen and to determine the suitable extender which will give higher conception rate. Semen was collected from five buffalo (5-6 years) bulls twice weekly using artificial vagina. Freshly collected semen samples were evaluated and ejaculates of at least 70% individual motility and 6x106 sperm /ml were pooled, divided into three parts, diluted with tris egg yolk, citrate egg yolk or skimmed milk extender and cooled slowly to 5oC. Semen samples were evaluated for individual motility, alive sperm, plasma and acrosomal membranes integrity as well as viability index. Conception rates of buffalo cows artificially inseminated with cooled buffalo semen were also estimated. The results of the present study revealed that semen extender and storage time exert a highly significant effects on the characteristics of cooled buffalo semen with the higher values recorded for tris egg yolk diluted samples. The conception rate of cooled buffalo semen was significantly (P<0.01) higher in tris (70%) diluted samples than skimmed milk (60%) and egg yolk citrate (50%) extended samples. It was concluded that the tris egg yolk extender is the most satisfactory buffering system to improve the post-cooling semen quality in buffalo and gave the best conception rate.

DOI

10.21608/kvmj.2008.116236

Keywords

Buffalo, cooled semen, Extender, fertility

Authors

First Name

Ismail

Last Name

El-Kon

MiddleName

Ismail

Affiliation

Theriogenology Department, Faculty of Veterinary Medicine, kafrelsheikh University, Kafr- Elsheikh -33516, Egypt.

Email

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City

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Orcid

-

First Name

El-Sayed

Last Name

Fattouh

MiddleName

Mohammed

Affiliation

Theriogenology Department, Faculty of Veterinary Medicine, kafrelsheikh University, Kafr- Elsheikh -33516, Egypt.

Email

-

City

-

Orcid

-

First Name

Samy

Last Name

Darwish

MiddleName

Anwer

Affiliation

Mehallat Mousa Buffalo Research Station, Animal Production Research Institute, Ministry of Agriculture, Egypt.

Email

-

City

-

Orcid

-

First Name

Fady

Last Name

Farag

MiddleName

samir

Affiliation

General Authority for Veterinary Services, Al-Gharbia, Province, Algarbia, Egypt.

Email

-

City

-

Orcid

-

Volume

6

Article Issue

2

Related Issue

16825

Issue Date

2008-10-01

Receive Date

2008-07-01

Publish Date

2008-10-01

Page Start

254

Page End

273

Print ISSN

1687-1456

Online ISSN

2682-2954

Link

https://kvmj.journals.ekb.eg/article_116236.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=116236

Order

12

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

EFFECT OF EXTENDERS ON THE CHARACTERISTIC AND FERTILITY OF COOLD BUFFALO SEMEN

Details

Type

Article

Created At

22 Jan 2023