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115955

DETECTION OF RETICULOENDOTHELIOSIS VIRUS AS CONTAMINANT OF FOWL POX VACCINES

Article

Last updated: 25 Dec 2024

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Subjects

-

Tags

Veterinary Medicine and Infectious Diseases “Veterinary Diseases".

Abstract

This study was performed to detect reticuloendotheliosis virus (REV) as a contaminant in fowl pox vaccines. A total of 30 fowl pox vaccine samples for the presences of REV using the in vitro a total and in vivo methods. In the in vitro test, the fowl pox vaccine samples were inoculated into chicken embryo fibroblast (CEF) cultures prepared from SPF embryonated chichen egg and examined by PCR test for detection  of REV. In the in vivo test,  each fowl pox vaccine sample was inoculated in five days old SPF chicks and kept under observation up to 12 weeks post inoculation (PI); serum samples were collected on 15th , 30th and 45th  day PI for the detection of antibodies against REV by commercial ELIZA kit, and tissue specimens were collected at 8th and 12th weeks PI for histopathological examination. The results revealed that: only one  imported vaccine sample gave positive results by PCR test a product of 291- bp was obtained by the vaccine sample. Serum samples collected from positive vaccine batch were tested for REV antibodies by ELIZA test and the sera were positive.  Histopathological examination of liver, spleen and bursa of fabricius revealed  the presence of tumor cells in the organs examined and these changes confirmed the results obtained by PCR and ELIZA test and indicated that the sample is contaminated with REV. The data clearly indicated that screening of all commercial poultry vaccine to be virus free is an important factor in assuring the biosafety of animal vaccines.

DOI

10.21608/kvmj.2008.115955

Keywords

reticuloendotheliosis virus, fowl pox vaccines

Authors

First Name

H. S.

Last Name

Abd El Hamid

MiddleName

-

Affiliation

Dept. of Avian and Aquatic Animal Medicine, Fac. of Vet. Med., Damanhour Branch, Alex Univ.

Email

-

City

-

Orcid

-

First Name

A. M.

Last Name

Awad

MiddleName

-

Affiliation

Dept. of Avian and Aquatic Animal Medicine, Fac. of Vet. Med., Edfina, Alex Univ.

Email

-

City

-

Orcid

-

First Name

A. A.

Last Name

Abou-Rawash

MiddleName

-

Affiliation

Dept. of Pathology and Parasitology, Fac. of Vet. Med., Damanhour Branch, Alex Univ.

Email

-

City

-

Orcid

-

First Name

H. F.

Last Name

Ellakany

MiddleName

-

Affiliation

Dept. of Avian and Aquatic Animal Medicine, Fac. of Vet. Med., Damanhour Branch, Alex Univ.

Email

-

City

-

Orcid

-

First Name

H. H.

Last Name

Ebrahim

MiddleName

-

Affiliation

Center Lab. for Evaluation of Veterinary Biologics, Abbassia , Cairo.

Email

-

City

-

Orcid

-

Volume

6

Article Issue

2

Related Issue

16825

Issue Date

2008-10-01

Receive Date

2008-07-01

Publish Date

2008-10-01

Page Start

114

Page End

138

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 6_Issue 2_Pages 114-138.pdf

PDF . 884.5kB

Link

https://kvmj.journals.ekb.eg/article_115955.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=115955

Order

5

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

DETECTION OF RETICULOENDOTHELIOSIS VIRUS AS CONTAMINANT OF FOWL POX VACCINES

Details

Type

Article

Created At

22 Jan 2023