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113553

MOLECULAR AND HISTOPATHOLOGICAL DETECTION OF RABBIT HEMORRHAGIC DISEASE VIRUS IN YOUNG RABBITS

Article

Last updated: 04 Jan 2025

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Subjects

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Tags

Veterinary Anatomy, Histology and Physiology “Veterinary Academic Studies".

Abstract

Calicivirus infection causes rabbit haemorrhagic disease (RHD) that kills more than 90% of adult animals, whereas young rabbits are naturally resistant to this viral disease. Interestingly, we have detected rabbit haemorrhagic disease virus (RHDV) in liver and intestinal tissues from suddenly died young rabbits (5-6 weeks old) in a remote private farm at Tukh, Kaluobia. Preliminary detection using haemagglutination (HA) test revealed a higher titer of HA antigen 27 and 28 in liver and intestine respectively using type O human erythrocytes. A genomic region encoding the capsid protein (VP60) within RHDV was identified by Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). The amplified cDNA gives size of approximately 538 bp. Also Liver and intestinal homogenates reacted positively by haemagglutination (HA) assays to different human blood groups (O, A, B, and AB) at 4 oC. Experimentally RHDV detected strain appears likely to be of high pathogenicity to young rabbits rather than adult. Histopathological examination showed acute necrotizing hepatic necrosis and crypt necrosis in the intestine. We conclude that a new RHDV strain infecting young rabbits (5-6 weeks old) was circulated and further characterization of the strain was required.

DOI

10.21608/kvmj.2011.113553

Keywords

Calicivirus infection, Rabbit Haemorrhagic Disease

Authors

First Name

E.M.

Last Name

El-Nahas

MiddleName

-

Affiliation

Department of virology, Faculty of Veterinary Medicine, Moshtohor, Benha University, Egypt.

Email

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City

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Orcid

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Volume

9

Article Issue

2

Related Issue

16744

Issue Date

2011-10-01

Receive Date

2011-07-01

Publish Date

2011-10-01

Page Start

179

Page End

193

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 9_Issue 2_Pages 179-193.pdf

PDF . 643.8kB

Link

https://kvmj.journals.ekb.eg/article_113553.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=113553

Order

10

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

MOLECULAR AND HISTOPATHOLOGICAL DETECTION OF RABBIT HEMORRHAGIC DISEASE VIRUS IN YOUNG RABBITS

Details

Type

Article

Created At

22 Jan 2023