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113550

USE OF POLYMERASE CHAIN REACTION FOR DIAGNOSIS OF BOVINE CORONAVIRUS IN DIARRHEIC CALVES

Article

Last updated: 04 Jan 2025

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Subjects

-

Tags

Veterinary Medicine and Infectious Diseases “Veterinary Diseases".

Abstract

Bovine Corona virus (BCV) is the main cause of diarrhea in many animal species world wide. Calves up to 3 months old can be affected by this virus. The importance of BCV in cattle industry is due to economic loss, treatment costs, and reduced growth rate in beef and dairy calves. This study dimed to examine 68 fecal samples(50 from diarrheic  and 18 from non diarrheic calves) by using reversed transcriptase-polymerase chain reaction (RT-PCR), antigen capture  enzymes linked Immunosorbent Assay (ELISA),direct Flourescent antibody test (FAT) and Haemagglutination and Haemagglutination inhibition technique  (HAT/HIT) for detecting of BCV in diarrheic and non diarrheic calves. The highest percentage of BCV in diarrheic calves by RT-PCR was 84 % then 76% by using BCV antigen capture ELISA then 62% by direct FAT and the lowest percentages was 56% by HIT. Also the apparent prevalence of BCV in non diarrheic calves were 44.4% by RT-PCR 27.7 by antigen capture ELISA, 22.2% by direct FAT and 11.1% by HIT respectively. These results suggested that RT-PCR is more sensitive   than other diagnostic methods to detect BCV, especially in subclinical cases.

DOI

10.21608/kvmj.2011.113550

Keywords

Bovine corona virus, diarrhea, calves

Authors

First Name

Nahed, A.

Last Name

Mahmoud

MiddleName

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Affiliation

Animal Health Research Institute, Dokki, Giza

Email

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City

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Orcid

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First Name

Hanaa, A. M.

Last Name

Ghoneim

MiddleName

-

Affiliation

Animal Reproduction Research Institute, Al-Harram, Giza

Email

-

City

-

Orcid

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First Name

Hala, A.

Last Name

Salem

MiddleName

-

Affiliation

Animal Health Research Institute, Dokki, Giza.

Email

-

City

-

Orcid

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Volume

9

Article Issue

2

Related Issue

16744

Issue Date

2011-10-01

Receive Date

2011-07-01

Publish Date

2011-10-01

Page Start

149

Page End

164

Print ISSN

1687-1456

Online ISSN

2682-2954

Article File

KVMJ_Volume 9_Issue 2_Pages 149-164.pdf

PDF . 659.2kB

Link

https://kvmj.journals.ekb.eg/article_113550.html

Detail API

https://kvmj.journals.ekb.eg/service?article_code=113550

Order

8

Type

Original Article

Type Code

1,064

Publication Type

Journal

Publication Title

Kafrelsheikh Veterinary Medical Journal

Publication Link

https://kvmj.journals.ekb.eg/

MainTitle

USE OF POLYMERASE CHAIN REACTION FOR DIAGNOSIS OF BOVINE CORONAVIRUS IN DIARRHEIC CALVES

Details

Type

Article

Created At

22 Jan 2023